Literature DB >> 17392448

Rates of and reasons for failure of commercial human immunodeficiency virus type 1 viral load assays in Brazil.

Jan Felix Drexler1, Luciano Kleber de Souza Luna, Celia Pedroso, Diana Brasil Pedral-Sampaio, Artur T L Queiroz, Carlos Brites, Eduardo M Netto, Christian Drosten.   

Abstract

We examined failures of commercial human immunodeficiency virus type 1 (HIV-1) viral load assays of 1,195 plasma samples from Brazilian patients. Assay failure was assumed for samples in which the virus was undetectable by commercial assay but which tested positive by real-time reverse transcription-PCR of the HIV-1 long terminal repeat (LTR) region or if the viral load differed by >2 log10 from that determined by LTR assay. Failure rates for Bayer Versant bDNA 3.0, Roche Amplicor Monitor v1.5, and bioMerieux NucliSens QT were 0.68, 0.47, and 4.33%, respectively. NucliSens may be inadequate for use in Brazil.

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Year:  2007        PMID： 17392448      PMCID： PMC1933096          DOI： 10.1128/JCM.00136-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

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  14 in total

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