Technical aspects of X-ray micro-computed tomography: initial experience of 27-microm resolution using Feldkamp cone-beam reconstruction.

The objective of this study was to introduce the technical utility of micro-computed tomography (CT) with 27-mum resolution by cone-beam CT algorithm. Whole-body micro-CT scans were performed to honeybee. Two- and three-dimensional image analyses were performed by originally developed and available open-source software for acquired images. The original contribution of this work is to describe the technical characteristics of the X-ray micro-CT system, keeping a small experimental insect in a unique condition. Micro-CT may be used as a rapid prototyping tool to research and understand the high-resolution system with Feldkamp cone-beam reconstruction.