Eicosanoid biosynthesis inhibitors increase the susceptibility of Lymantria dispar to nucleopolyhedrovirus LdMNPV.

Eighteen pharmaceutical inhibitors of eicosanoid biosynthesis were tested for their effects on gypsy moth, Lymantria dispar and its susceptibility to the nucleopoly-hedrovirus LdMNPV. None of the inhibitors tested had any detrimental effects upon larval growth and development. Treatment with nine inhibitor/NPV combinations (e.g., bromophenacylbromide, clotrimazole, dexamethasone, esculetin, flufenamic acid, indomethacin, nimesulide, sulindac, tolfenamic acid) resulted in 3.5- to 6.6-fold reductions in LC(50)s. Larvae treated with several other COX inhibitors did not yield significant LC(50) reductions. We infer that eicosanoids act in insect defense responses to viral infection. Eicosanoids may act at three levels of insect immune reactions to viral infection, organismal (febrile response), cellular (hemocytic microaggregation, nodulation and plasmatocytes spreading reactions) and intracellular level (mechanisms responsible for insect permissiveness to viral replication).