Study of the sensitization of tetradecyl benzyl dimethyl ammonium chloride for spectrophotometric determination of dopamine hydrochloride using sodium 1,2-naphthoquinone-4-sulfonate as the chemical derivative chromogenic reagent.

A new method has been established for the determination of dopamine hydrochloride (DPH) using sodium 1,2-naphthoquinone-4-sulfonate (NQS) and tetradecyl benzyl dimethyl ammonium chloride (Zeph). This method is based on the formation of a pink compound from the reaction of DPH, sodium 1,2-naphthoquinone-4-sulfonate and Zeph. The condensation reaction proceeds quantitatively in pH 9.40 buffer solution. The maximum absorption wavelength and the value of epsilon491 were 491 nm, and 7.51x10(3) l mol(-1) cm(-1), respectively, when the stoichiometric ratio of the reaction was 1:1:1. Beer's law was obeyed in the range of 0.16-40 mg l(-1) of DPH. The data have been filled to a linear regression equation A=0.5781+0.0254 C (mg l(-1)), with a correlation coefficient of 0.9993. The detection limit is 0.12 mg l(-1), R.S.D. is 0.64% (40 mg l(-1), n=11), and average recovery is over 99.7%. This paper further improves the determination of DPH compared to the previous methods. The kinetic property and reaction mechanism have also been discussed. This proposed method has been successfully applied to the determination of DPH in injection and biological samples with satisfactory results.