| Literature DB >> 17384812 |
Erica Fugikaha1, Patrícia Aparecida Fornazari, Roberta de Souza Rodrigues Penhalbel, Alexandre Lorenzetti, Roberto Duarte Maroso, Juvanete Távora Amoras, Ana Sueli Saraiva, Rita Uchôa da Silva, Cláudia Regina Bonini-Domingos, Luiz Carlos de Mattos, Andréa Regina Baptista Rossit, Carlos Eugênio Cavasini, Ricardo Luiz Dantas Machado.
Abstract
The transmission of malaria in Brazil is heterogeneous throughout endemic areas and the presence of asymptomatic Plasmodium sp. carriers (APCs) in the Brazilian Amazon has already been demonstrated. Malaria screening in blood banks is based on the selection of donors in respect to possible risks associated with travel or residence, clinical evidence and/or inaccurate diagnostic methods thereby increasing the probability of transfusion-transmitted infection. We evaluated the frequency of APCs in four blood services in distinct areas of the Brazilian Amazon region. DNA was obtained from 400 human blood samples for testing using the phenol-chloroform method followed by a nested-PCR protocol with species-specific primers. The positivity rate varied from 1 to 3% of blood donors from the four areas with an average of 2.3%. All positive individuals had mixed infections for Plasmodium vivax and Plasmodium falciparum. No significant differences in the results were detected among these areas; the majority of cases originated from the transfusion centres of Porto Velho, Rondônia State and Macapá, Amapá State. Although it is still unclear whether APC individuals may act as reservoirs of the parasite, efficient screening of APCs and malaria patients in Brazilian blood services from endemic areas needs to be improved.Entities:
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Year: 2007 PMID： 17384812 DOI： 10.1590/s0036-46652007000100001
Source DB: PubMed Journal: Rev Inst Med Trop Sao Paulo ISSN： 0036-4665 Impact factor: 1.846