| Literature DB >> 17382903 |
Hisashi Hatanaka1, Shuji Takada, Young Lim Choi, Shin-ichiro Fujiwara, Manabu Soda, Munehiro Enomoto, Kentaro Kurashina, Hideki Watanabe, Yoshihiro Yamashita, Kentaro Sugano, Hiroyuki Mano.
Abstract
Colorectal cancer (CRC) is one of the leading causes of cancer death in humans. In order to identify novel cancer-promoting genes in CRC, we here constructed a retroviral cDNA expression library from a CRC cell line RKO, and used it for a focus formation assay with mouse 3T3 fibroblasts, leading to the identification of 42 independent cDNAs. One of such cDNAs turned out to encode purinergic receptor P2Y, G-protein coupled, 2 (P2RY2). The oncogenic potential of P2RY2 was confirmed in vitro with the focus formation assay as well as soft agar-growth assay, and also in vivo with a tumorigenicity assay in nude mice. While our P2RY2 cDNA encodes a protein with two amino-acid substitutions compared to the reported one, we have confirmed that the wild-type P2RY2 has a strong transforming potential as well. These results indicate an unexpected role of P2RY2 in the carcinogenesis of human cancers.Entities:
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Year: 2007 PMID： 17382903 DOI： 10.1016/j.bbrc.2007.03.048
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575