Literature DB >> 17381871

Utilizing a polymerase chain reaction method for the detection of Toxocara canis and T. cati eggs in soil.

R Fogt-Wyrwas1, W Jarosz, H Mizgajska-Wiktor.   

Abstract

A polymerase chain reaction (PCR) technique has been used for the differentiation of T. canis and T. cati eggs isolated from soil and previously identified from microscopical observations. The method, using specific primers for the identification of the two Toxocara species, was assessed in both the field and laboratory. Successful results were obtained when only a single or large numbers of eggs were recovered from 40 g soil samples. The method is sensitive, allows analysis of material independent of the stage of egg development and can be adapted for the recovery of other species of parasites from soil.

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Year:  2007        PMID: 17381871     DOI: 10.1017/S0022149X07241872

Source DB:  PubMed          Journal:  J Helminthol        ISSN: 0022-149X            Impact factor:   2.170


  7 in total

1.  Prevalence and molecular characterization of zoonotic helminths in dogs.

Authors:  G Suganya; K Porteen; M Sekar; A Sangaran
Journal:  J Parasit Dis       Date:  2018-12-17

2.  Molecular and microscopic detection of natural and experimental infections of Toxocara vitulorum in bovine milk.

Authors:  Amira Dewair; Mohamed Bessat
Journal:  PLoS One       Date:  2020-05-20       Impact factor: 3.240

Review 3.  Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook.

Authors:  Miao-Han Deng; Lan-Yi Zhong; Okanurak Kamolnetr; Yanin Limpanont; Zhi-Yue Lv
Journal:  Infect Dis Poverty       Date:  2019-03-25       Impact factor: 4.520

4.  Intestinal Parasites of Pets and Other House-kept Animals in Moscow.

Authors:  O P Kurnosova; M V Arisov; I M Odoyevskaya
Journal:  Helminthologia       Date:  2019-06-01       Impact factor: 1.184

5.  Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples.

Authors:  Jean-Francois Durant; Leonid M Irenge; Renata Fogt-Wyrwas; Catherine Dumont; Jean-Pierre Doucet; Bernard Mignon; Bertrand Losson; Jean-Luc Gala
Journal:  Parasit Vectors       Date:  2012-12-07       Impact factor: 3.876

6.  PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.

Authors:  Shahram Khademvatan; Fakher Rahim; Mahdi Tavalla; Rahman Abdizadeh; Mahmoud Hashemitabar
Journal:  PLoS One       Date:  2013-06-03       Impact factor: 3.240

Review 7.  Environmental contamination by canine geohelminths.

Authors:  Donato Traversa; Antonio Frangipane di Regalbono; Angela Di Cesare; Francesco La Torre; Jason Drake; Mario Pietrobelli
Journal:  Parasit Vectors       Date:  2014-02-13       Impact factor: 3.876

  7 in total

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