| Literature DB >> 17381727 |
Huaizhi Dou1, Min Zhang, Yu Zhang, Chunhua Yin.
Abstract
Monomethoxypoly(ethylene glycol) 5000 (mPEG5K) and 2000 (mPEG2K) were activated to produce mPEG-aldehyde with content over 80%. Effects of reaction time, pH and initial molar ratio of mPEG-aldehyde to insulin on synthesis of PEGylated insulin were studied, taking mPEG5K-aldehyde as a model. On the optimized condition, approximately 90% of product was mono-PEGylated insulin. Mono-PEGylated insulin was purified by ion-exchange chromatography. The purity of each component was analysed by RP-HPLC and the results showed an efficient purification. Matrix assistant laser desorption ionization time-of-flight mass spectra demonstrated that the modification site of mono-PEGylated insulin was PheB1. The biological activity of all PEGylated insulin was also evaluated.Entities:
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Year: 2007 PMID： 17381727 DOI： 10.1111/j.1747-0285.2007.00479.x
Source DB: PubMed Journal: Chem Biol Drug Des ISSN： 1747-0277 Impact factor: 2.817