Literature DB >> 17381622

Sterility testing of hematopoietic progenitor cell products: a single-institution series of culture-positive rates and successful infusion of culture-positive products.

Douglas J Padley1, Allan B Dietz, Dennis A Gastineau.   

Abstract

BACKGROUND: Administration of culture-positive hematopoietic progenitor cells (HPCs) causing adverse events has been a hypothesized yet largely unmeasured risk of the clinical practice of HPC transplantation. To enhance patient safety, the FDA has issued regulations prohibiting the use of culture-positive HPCs. Numerous studies have reported the infusion of culture-positive HPCs; however, the low frequency of adverse events prevents accurate determination of this risk. STUDY DESIGN AND METHODS: Product culture results and clinical outcomes from January 1998 through March 2006 representing 7233 HPC collections for 2118 transplants at a single institution were reviewed.
RESULTS: A total of 119 units of HPCs (1.6%) intended for 95 patients were culture-positive. Of the 69 patients transplanted with culture-positive HPCs, 5 received products with cultures pending, and 64 received products with the positive culture results known. One of 69 patients had a new positive blood culture 5 days after infusion with the same species as the product. There was not a clinically relevant difference in the rate of infusion-related symptoms reported for patients who received culture-positive products compared to all infusions. The survival of patients who received culture-positive products (n = 69) was not different from all HPC recipients (n = 2046; p = 0.419).
CONCLUSION: No infusion-related risks of culture-positive HPCs to patient safety were identified. Our data suggest that the decision to use culture-positive HPCs must be made in the context of the global risks associated with transplants such as remobilization, replacement product availability, and the nature of the organism.

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Year:  2007        PMID: 17381622     DOI: 10.1111/j.1537-2995.2007.01165.x

Source DB:  PubMed          Journal:  Transfusion        ISSN: 0041-1132            Impact factor:   3.157


  4 in total

1.  Effects of storage temperature on hematopoietic stability and microbial safety of BM aspirates.

Authors:  S Hahn; W Sireis; K Hourfar; D Karpova; K Dauber; V A J Kempf; E Seifried; M Schmidt; H Bönig
Journal:  Bone Marrow Transplant       Date:  2013-11-04       Impact factor: 5.483

2.  Guidelines for preventing infectious complications among hematopoietic cell transplantation recipients: a global perspective.

Authors:  Marcie Tomblyn; Tom Chiller; Hermann Einsele; Ronald Gress; Kent Sepkowitz; Jan Storek; John R Wingard; Jo-Anne H Young; Michael J Boeckh; Michael A Boeckh
Journal:  Biol Blood Marrow Transplant       Date:  2009-10       Impact factor: 5.742

Review 3.  Sterility Testing for Cellular Therapies: What Is the Role of the Clinical Microbiology Laboratory?

Authors:  James E T Gebo; Anna F Lau
Journal:  J Clin Microbiol       Date:  2020-06-24       Impact factor: 5.948

4.  Use of 27G needles improves sensitivity and performance of ATCC anaerobe reference microorganism detection in BacT/Alert system.

Authors:  Salvatore Pasqua; Giampiero Vitale; Anna Pasquariello; Bruno Douradinha; Fabio Tuzzolino; Francesca Cardinale; Chiara Cusimano; Chiara Di Bartolo; Pier Giulio Conaldi; Danilo D'Apolito
Journal:  Mol Ther Methods Clin Dev       Date:  2021-01-26       Impact factor: 6.698

  4 in total

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