K Park1, S Han, E Shin, H J Kim, J Y Kim. 1. Department of Pathology, Inje University Sanggye Paik Hospital, 761-1 Sanggye-Dong, Nowon-Gu, Seoul, South Korea.
Abstract
AIMS: To measure the EGFR gene amplification and protein expression, and to compare the EGFR expression with HER2 expression similarly measured. METHODS: EGFR gene amplification was assayed by fluorescence in situ hybridization and its protein expression was evaluated by immunohistochemistry on tissue microarray of 165 consecutive invasive breast cancers. RESULTS: EGFR protein overexpression was observed in 20.6% of the 165 studied cases, but gene amplification was observed in 7.9% only. EGFR protein was expressed in 33.3% of HER2-amplified tumors, and in only 16.3% of HER2 non-amplified tumors. EGFR expression significantly increased in HER2 gene-amplified and protein-expressing tumors as well. On survival analysis, EGFR expression was a significant prognostic factor for the disease-free and overall survival of the patients. CONCLUSIONS: EGFR protein expression was independent of EGFR gene amplification status, whereas it was intimately associated with HER2 amplification and overexpression in breast cancer.
AIMS: To measure the EGFR gene amplification and protein expression, and to compare the EGFR expression with HER2 expression similarly measured. METHODS:EGFR gene amplification was assayed by fluorescence in situ hybridization and its protein expression was evaluated by immunohistochemistry on tissue microarray of 165 consecutive invasive breast cancers. RESULTS:EGFR protein overexpression was observed in 20.6% of the 165 studied cases, but gene amplification was observed in 7.9% only. EGFR protein was expressed in 33.3% of HER2-amplified tumors, and in only 16.3% of HER2 non-amplified tumors. EGFR expression significantly increased in HER2 gene-amplified and protein-expressing tumors as well. On survival analysis, EGFR expression was a significant prognostic factor for the disease-free and overall survival of the patients. CONCLUSIONS:EGFR protein expression was independent of EGFR gene amplification status, whereas it was intimately associated with HER2 amplification and overexpression in breast cancer.
Authors: Aleksandra Sobczyńska-Rak; Beata Żylińska; Łukasz Jarosz; Adam Brodzki; Marcin Tatara Journal: In Vivo Date: 2018 Sep-Oct Impact factor: 2.155
Authors: Cathy B Moelans; Roel A de Wegers; Hanneke N Monsuurs; Anoek H J Maess; Paul J van Diest Journal: Cell Oncol (Dordr) Date: 2011-05-06 Impact factor: 6.730