Laboratory efficacy of metabolites of Lagenidium giganteum (Couch) on Anopheles stephensi (Liston) after filterations by Column Chromatography.

This study was conducted in laboratory to evaluate the efficacy of filtered extracellular metabolites of Lagenidium giganteum against all the four instars of An. stephensi larvae. Fungal colonies have been cultured in PYG broth and after 15 days of culturing the fungus, metabolites have been filtered twice by whatman filter paper. These metabolites were again filtered by column chromatography and by rang syringe filters. Filtered metabolites were then used against all instars of An. stephensi larvae. The bioassays were conducted at five significantly different concentrations (1.68, 1.99, 2.17, 2.30, 2.40 ppm). The results suggest significant mortality on first three instar larvae and very low on fourth instar larvae.