| Literature DB >> 17368572 |
Mohsen Hosseinkhani1, Koji Hasegawa, Koh Ono, Teruhisa Kawamura, Tomohide Takaya, Tatsuya Morimoto, Hiromichi Wada, Akira Shimatsu, Susana Gonzalez Prat, Hirofumi Suemori, Norio Nakatsuji, Toru Kita.
Abstract
Human embryonic stem (ES) cell lines are one of the possible sources of cardiac myocytes to be transplanted in patients with end-staged heart failure. However, prior to the application of human of ES cells for heart failure therapy, it is critical to validate their clinical use in large animals such as primates. Cynomolgus monkey ES cells have similar properties to human ES cells and can be used for primate studies. We demonstrate that 24-h stimulation by a histone deacetylase inhibitor, trichostatin A (TSA) facilitated myocardial differentiation of monkey ES cells with embryonic bodies that were seeded on gelatin-coated dishes. TSA-induced acetylating of histone-3/4 and expression of p300, one of the intrinsic histone acetyltransferases. Thus, such induction as well as inhibition of histone deacetylase may be involved in TSA-induced differentiation of cynomolgus monkey ES cells into cardiomyocytes.Entities:
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Year: 2007 PMID： 17368572 DOI： 10.1016/j.bbrc.2007.02.151
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575