| Literature DB >> 17359946 |
Jingbao Li1, Feng Han, Xinzhi Lu, Xiaoyan Fu, Cuiping Ma, Yan Chu, Wengong Yu.
Abstract
In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant beta-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and (13)C NMR spectroscopy.Entities:
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Year: 2007 PMID： 17359946 DOI： 10.1016/j.carres.2007.02.008
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104