Lin Zheng1, Yida Yang, Lu Guocai, C David Pauza, Maria S Salvato. 1. Department of Infectious Diseases, The First Affiliated Hospital, Medical School, Key Laboratory of Infectious Diseases of Chinese Ministry of Public Health, Zhejiang University, Hangzhou, Zhejiang, PR China.
Abstract
OBJECTIVE: To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS: Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexin V and 7-AAD staining were used to detect apoptosis of monocytes induced by TRAIL. RESULTS: HIV Tat protein increased Bcl-2 expression in human monocytes in a dose-dependent manner. Annexin V staining showed that 51.54% of monocytes underwent apoptosis after being treated with 100 ng/ml recombinant TRAIL. When monocytes were prestimulated with HIV Tat, only 15.46% of monocytes underwent apoptosis. This effect can be inhibited by polyclonal anti-Tat serum. 7-AAD staining showed similar results. CONCLUSION: HIV Tat protein increases Bcl-2 expression in monocytes which inhibited apoptosis induced by TRAIL. HIV Tat protein may play an important role in the mechanisms of HIV-persistent infection in monocytes. (c) 2007 S. Karger AG, Basel.
OBJECTIVE: To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS: Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexin V and 7-AAD staining were used to detect apoptosis of monocytes induced by TRAIL. RESULTS: HIV Tat protein increased Bcl-2 expression in human monocytes in a dose-dependent manner. Annexin V staining showed that 51.54% of monocytes underwent apoptosis after being treated with 100 ng/ml recombinant TRAIL. When monocytes were prestimulated with HIV Tat, only 15.46% of monocytes underwent apoptosis. This effect can be inhibited by polyclonal anti-Tat serum. 7-AAD staining showed similar results. CONCLUSION: HIV Tat protein increases Bcl-2 expression in monocytes which inhibited apoptosis induced by TRAIL. HIV Tat protein may play an important role in the mechanisms of HIV-persistent infection in monocytes. (c) 2007 S. Karger AG, Basel.
Authors: V Cantaluppi; M C Deregibus; L Biancone; I Deambrosis; B Bussolati; A Albini; G Camussi Journal: Int J Immunopathol Pharmacol Date: 2006 Jan-Mar Impact factor: 3.219
Authors: J J Lum; A A Pilon; J Sanchez-Dardon; B N Phenix; J E Kim; J Mihowich; K Jamison; N Hawley-Foss; D H Lynch; A D Badley Journal: J Virol Date: 2001-11 Impact factor: 5.103
Authors: Marion MacFarlane; Nicholas Harper; Roger T Snowden; Martin J S Dyer; Georgina A Barnett; J Howard Pringle; Gerald M Cohen Journal: Oncogene Date: 2002-10-03 Impact factor: 9.867
Authors: Yida Yang; Ilia Tikhonov; Tracy J Ruckwardt; Mahmoud Djavani; Juan Carlos Zapata; C David Pauza; Maria S Salvato Journal: J Virol Date: 2003-06 Impact factor: 5.103
Authors: Nathan W Cummins; Amy M Sainski-Nguyen; Sekar Natesampillai; Fatma Aboulnasr; Scott Kaufmann; Andrew D Badley Journal: J Virol Date: 2017-05-12 Impact factor: 5.103
Authors: Malavika S Giri; Michael Nebozyhn; Andrea Raymond; Bethsebah Gekonge; Aidan Hancock; Shenoa Creer; Calen Nicols; Malik Yousef; Andrea S Foulkes; Karam Mounzer; Jane Shull; Guido Silvestri; Jay Kostman; Ronald G Collman; Louise Showe; Luis J Montaner Journal: J Immunol Date: 2009-04-01 Impact factor: 5.422
Authors: Francesc Cunyat; Jennifer N Rainho; Brian West; Louise Swainson; Joseph M McCune; Mario Stevenson Journal: J Virol Date: 2016-06-24 Impact factor: 5.103