Wanlop Weecharangsan1, Praneet Opanasopit, Robert J Lee. 1. College of Pharmacy, NCI OSU Comprehensive Cancer Center, NSF Nanoscales Science and Engineering Center (NSEC) for Affordable Nanoengineering of Polymeric Biomedical Devices (CANPBD), The Ohio State University, Columbus, 43210, USA.
Abstract
BACKGROUND: The aim of this study was to investigate the transfection efficiency of cationic vectors (polyethylenimine; PEI25K and lipofectamine), electroporation and their combination in the human cancer cell lines, Raji human lymphoma and KB human oral carcinoma. MATERIALS AND METHODS: Raji human lymphoma and KB human oral carcinoma cell lines were transfected with pcDNA3-CMV-Luc at various N/P ratios of cationic vectors and voltages of electroporation, as well as with a combination of the cationic vectors and electroporation. RESULTS: The major findings were: (a) cationic vectors or electroporation alone increased transfection efficiency; (b) cationic vectors inhibited the transfection efficiency by electroporation. CONCLUSION: Our results demonstrate that cationic vectors and electroporation are feasible and efficient in transfecting human cancer cell lines. However, a combination of cationic vectors and electroporation is ineffective.
BACKGROUND: The aim of this study was to investigate the transfection efficiency of cationic vectors (polyethylenimine; PEI25K and lipofectamine), electroporation and their combination in the humancancer cell lines, Raji humanlymphoma and KB humanoral carcinoma. MATERIALS AND METHODS: Raji humanlymphoma and KB humanoral carcinoma cell lines were transfected with pcDNA3-CMV-Luc at various N/P ratios of cationic vectors and voltages of electroporation, as well as with a combination of the cationic vectors and electroporation. RESULTS: The major findings were: (a) cationic vectors or electroporation alone increased transfection efficiency; (b) cationic vectors inhibited the transfection efficiency by electroporation. CONCLUSION: Our results demonstrate that cationic vectors and electroporation are feasible and efficient in transfecting humancancer cell lines. However, a combination of cationic vectors and electroporation is ineffective.