OBJECTIVE: The purpose of this study was to examine the association between dietary intake of phytoestrogens estimated by a food frequency questionnaire (FFQ) with urinary metabolites. METHODS: Participants were 26 premenopausal, Caucasian women aged 25 to 42 years. Dietary intake of isoflavones (genistein and daidzein) and lignans (secoisolariciresinol and matairesinol) were estimated by a 53-item interviewer-administered FFQ on two occasions, reflecting 'habitual' (previous 2 months) and 'recent' (previous 2 days) dietary intake. Isoflavone (genistein, daidzein) and lignan (enterolactone, enterodiol and secoisolariciresinol) concentrations were measured in 24-hour urine samples by gas chromatography-mass spectrometry. Correlations between FFQ (habitual and recent, separately) and urinary metabolite values were assessed using Spearman correlation coefficients. RESULTS: Mean habitual isoflavone and lignan intakes were 13.7 mg/day and 13.8 mg/day, respectively. Mean urinary concentrations of isoflavones and lignans were 17.4 micromol/day and 20.6 micromol/day, respectively. Recent and habitual isoflavone intakes were correlated with urinary excretion of metabolites (r = 0.64, p < 0.001 and r = 0.54, p = 0.004, respectively). Urinary excretion of lignans was also modestly correlated with recent and habitual lignan intakes (r = 0.46, p = 0.02 and r = 0.40, p = 0.05, respectively). CONCLUSIONS: Our results support the use of this FFQ as a measure of dietary isoflavone and lignan intake in epidemiological studies.
OBJECTIVE: The purpose of this study was to examine the association between dietary intake of phytoestrogens estimated by a food frequency questionnaire (FFQ) with urinary metabolites. METHODS:Participants were 26 premenopausal, Caucasian women aged 25 to 42 years. Dietary intake of isoflavones (genistein and daidzein) and lignans (secoisolariciresinol and matairesinol) were estimated by a 53-item interviewer-administered FFQ on two occasions, reflecting 'habitual' (previous 2 months) and 'recent' (previous 2 days) dietary intake. Isoflavone (genistein, daidzein) and lignan (enterolactone, enterodiol and secoisolariciresinol) concentrations were measured in 24-hour urine samples by gas chromatography-mass spectrometry. Correlations between FFQ (habitual and recent, separately) and urinary metabolite values were assessed using Spearman correlation coefficients. RESULTS: Mean habitual isoflavone and lignan intakes were 13.7 mg/day and 13.8 mg/day, respectively. Mean urinary concentrations of isoflavones and lignans were 17.4 micromol/day and 20.6 micromol/day, respectively. Recent and habitual isoflavone intakes were correlated with urinary excretion of metabolites (r = 0.64, p < 0.001 and r = 0.54, p = 0.004, respectively). Urinary excretion of lignans was also modestly correlated with recent and habitual lignan intakes (r = 0.46, p = 0.02 and r = 0.40, p = 0.05, respectively). CONCLUSIONS: Our results support the use of this FFQ as a measure of dietary isoflavone and lignan intake in epidemiological studies.
Authors: Carol J Fabian; Bruce F Kimler; Carola M Zalles; Jennifer R Klemp; Brian K Petroff; Qamar J Khan; Priyanka Sharma; Kenneth D R Setchell; Xueheng Zhao; Teresa A Phillips; Trina Metheny; Jennifer R Hughes; Hung-Wen Yeh; Karen A Johnson Journal: Cancer Prev Res (Phila) Date: 2010-08-19
Authors: Angela M Leung; Andrew Lamar; Xuemei He; Lewis E Braverman; Elizabeth N Pearce Journal: J Clin Endocrinol Metab Date: 2011-05-25 Impact factor: 5.958
Authors: Suzan L Carmichael; Mary E Cogswell; Chen Ma; Amparo Gonzalez-Feliciano; Richard S Olney; Adolfo Correa; Gary M Shaw Journal: Am J Epidemiol Date: 2013-06-09 Impact factor: 4.897