BACKGROUND: Fibroblast growth factor-2 (FGF-2) has a role in the angiogenesis induced by renal carcinoma. MATERIALS AND METHODS: Blockage of FGF-2 by an antisense oligonucleotide (ASO) or by a mouse neutralizing anti-human FGF-2 monoclonal antibody (anti-FGF-2-mAb) was evaluated on a cell line isolated from a renal carcinoma bone metastasis (CRBM-1990), on Caki-1 and ACHN cells. Cocultures of endothelial cells and ASO- or mAb-treated carcinoma lines were investigated. RESULTS: Anti-FGF-2-mAb treatment induced a 33% reduction of FGF-2 released by ACHN, a 31% reduction of FGF-2 released by Caki-1, and a 70% reduction of FGF-2 released by CRBM-1990. ASO treatment did not inhibit endothelial cell proliferation. In contrast, anti-FGF-2-mAb significantly decreased endothelial cells proliferation induced by ACHN and CRBM-1990. The inhibition of endothelial cell growth was reverted by recombinant FGF-2. CONCLUSION: Modulation of FGF-2 production by renal cell carcinoma with a blocking mAb produced a significant inhibition of endothelial cell growth.
BACKGROUND:Fibroblast growth factor-2 (FGF-2) has a role in the angiogenesis induced by renal carcinoma. MATERIALS AND METHODS: Blockage of FGF-2 by an antisense oligonucleotide (ASO) or by a mouse neutralizing anti-humanFGF-2 monoclonal antibody (anti-FGF-2-mAb) was evaluated on a cell line isolated from a renal carcinoma bone metastasis (CRBM-1990), on Caki-1 and ACHN cells. Cocultures of endothelial cells and ASO- or mAb-treated carcinoma lines were investigated. RESULTS: Anti-FGF-2-mAb treatment induced a 33% reduction of FGF-2 released by ACHN, a 31% reduction of FGF-2 released by Caki-1, and a 70% reduction of FGF-2 released by CRBM-1990. ASO treatment did not inhibit endothelial cell proliferation. In contrast, anti-FGF-2-mAb significantly decreased endothelial cells proliferation induced by ACHN and CRBM-1990. The inhibition of endothelial cell growth was reverted by recombinant FGF-2. CONCLUSION: Modulation of FGF-2 production by renal cell carcinoma with a blocking mAb produced a significant inhibition of endothelial cell growth.
Authors: C G Stoehr; R Stoehr; A Hartmann; F Hofstaedter; K Junker; H Blaszyk; W F Wieland; W Otto; S Denzinger; B Walter Journal: J Cancer Res Clin Oncol Date: 2011-12-28 Impact factor: 4.553
Authors: Tram Anh Tran; Hon Sing Leong; Andrea Pavia-Jimenez; Slavic Fedyshyn; Juan Yang; Blanka Kucejova; Sharanya Sivanand; Patrick Spence; Xian-Jin Xie; Samuel Peña-Llopis; Nicholas Power; James Brugarolas Journal: Mol Cell Biol Date: 2016-06-15 Impact factor: 4.272
Authors: Kelly Aukes; Cynthia Forsman; Nicholas J Brady; Kristina Astleford; Nicholas Blixt; Deepali Sachdev; Eric D Jensen; Kim C Mansky; Kathryn L Schwertfeger Journal: PLoS One Date: 2017-10-02 Impact factor: 3.240