OBJECTIVE: We conducted this study to evaluate the association of cytokine genotypes and sputum concentrations on longitudinal decline in lung function in firefighters. METHODS: In 67 firefighters with at least four pulmonary function tests, DNA was analyzed for functional polymorphisms of interleukin (IL)-1beta, IL-1 receptor antagonist (IL-1RA), IL-8, IL-10, tumor necrosis factor-alpha (TNF-alpha) genes, and sputum evaluated for cytokine concentration by ELISA. RESULTS: The annual rate of FEV1 decline was greater in firefighters with TT genotypes at IL-10 (-819) (P=0.009) and with CT or TT genotypes at IL-1RA (2018) (P=0.050). These genotypes were not associated with concentrations of sputum cytokine, but increased IL-1RA was associated with a slower rate of FEV1 decline (P=0.025), as was increased sputum macrophage count (P=0.002). CONCLUSIONS: Cytokine genotypes were associated with the rate of FEV1 decline but did not alter concentrations of sputum cytokine. Increased sputum IL-1RA may be protective.
OBJECTIVE: We conducted this study to evaluate the association of cytokine genotypes and sputum concentrations on longitudinal decline in lung function in firefighters. METHODS: In 67 firefighters with at least four pulmonary function tests, DNA was analyzed for functional polymorphisms of interleukin (IL)-1beta, IL-1 receptor antagonist (IL-1RA), IL-8, IL-10, tumor necrosis factor-alpha (TNF-alpha) genes, and sputum evaluated for cytokine concentration by ELISA. RESULTS: The annual rate of FEV1 decline was greater in firefighters with TT genotypes at IL-10 (-819) (P=0.009) and with CT or TT genotypes at IL-1RA (2018) (P=0.050). These genotypes were not associated with concentrations of sputum cytokine, but increased IL-1RA was associated with a slower rate of FEV1 decline (P=0.025), as was increased sputum macrophage count (P=0.002). CONCLUSIONS: Cytokine genotypes were associated with the rate of FEV1 decline but did not alter concentrations of sputum cytokine. Increased sputum IL-1RA may be protective.
Authors: Yu Wang; Karey Shumansky; Don D Sin; S F Paul Man; Loubna Akhabir; John E Connett; Nicholas R Anthonisen; Peter D Paré; Andrew J Sandford; Jian-Qing He Journal: Int J Clin Exp Pathol Date: 2015-10-01
Authors: Joana V Barbosa; Rafael A O Nunes; Maria C M Alvim-Ferraz; Fernando G Martins; Sofia I V Sousa Journal: Int J Environ Res Public Health Date: 2022-01-05 Impact factor: 3.390