| Literature DB >> 17350636 |
Susanne Baldermann1, Annika Reinhard, Nils Köhler, Peter Fleischmann.
Abstract
Preparative HSCCC (high-speed counter-current chromatography) could be applied for the isolation of 9'-cis-neoxanthin from a crude carotenoid extract of fresh spinach leaves. The separation was performed on a Pharma-Tech Research Corp. CCC 1000 with a solvent system composed of hexane:ethanol:water at a volume ratio of 5:5:4.5 at a flow rate of 3 mL/min and at 850 rpm, using the lower phase as mobile phase. 9'-cis-neoxanthin with a purity of up to 94% could be obtained with a single HSCCC purification step of the crude carotenoid extract.Entities:
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Year: 2007 PMID： 17350636 DOI： 10.1016/j.chroma.2007.02.064
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759