| Literature DB >> 17350223 |
Min Zheng1, Qi Liu, Ningyi Jin, Jiangang Guo, Xia Huang, Huaming Li, Wei Zhu, Yi Xiong.
Abstract
A duplex polymerase chain reaction (PCR) was developed and optimized for simultaneous detection and differentiation of Capripoxvirus (CPV) (including goat pox virus and sheep pox virus) and orf virus (ORFV). Two sets of specific oligonucleotide primers were designed and used for CPV and ORFV, respectively. The duplex PCR DNA products, which consisted of fragments of 413 bp for A29L gene of CPV, and 708 bp for P55 gene of ORFV, were visualized by gel electrophoresis. The developed assay was found to be highly specific and sensitive with a detection limit of 1 plaque forming unit (pfu) for both CPV and CPV.Entities:
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Year: 2007 PMID： 17350223 DOI： 10.1016/j.mcp.2007.01.005
Source DB: PubMed Journal: Mol Cell Probes ISSN： 0890-8508 Impact factor: 2.365