OBJECTIVE: To report the survival rate of oocytes and the rate of successful pregnancies obtained from super-rapid cooling of oocytes using slush nitrogen (SN(2)). DESIGN: Prospective clinical research. SETTING: A university-affiliated hospital. PATIENT(S): Twenty-eight infertile women who underwent 30 cycles of IVF-ET using previously vitrified oocytes. INTERVENTION(S): Oocytes were vitrified by super-rapid cooling using SN(2). MAIN OUTCOME MEASURE(S): Morphological normality of thawed oocytes and clinical outcome. RESULT(S): In 30 cycles of ovarian stimulation for IVF, 364 surplus oocytes from 28 patients were vitrified using SN(2). Three hundred two (85.1% +/- 2.9%) of the oocytes survived after warming. Fertilization and cleavage rates were 77.4% +/- 3.5% (168/218) and 94.3% +/- 2.1% (158/168), respectively. Thirteen pregnancies (43.3%) resulted from 30 uterine transfers of 120 embryos with an implantation rate of 14.2% (17/120). There were no differences between the pregnancy rate after vitrification/warming and that obtained from routine noncryopreserved oocytes. CONCLUSION(S): The present report suggests that super-rapid cooling may improve the clinical efficacy of human oocyte vitrification and may be a valuable tool for human assisted reproductive technologies.
OBJECTIVE: To report the survival rate of oocytes and the rate of successful pregnancies obtained from super-rapid cooling of oocytes using slush nitrogen (SN(2)). DESIGN: Prospective clinical research. SETTING: A university-affiliated hospital. PATIENT(S): Twenty-eight infertile women who underwent 30 cycles of IVF-ET using previously vitrified oocytes. INTERVENTION(S): Oocytes were vitrified by super-rapid cooling using SN(2). MAIN OUTCOME MEASURE(S): Morphological normality of thawed oocytes and clinical outcome. RESULT(S): In 30 cycles of ovarian stimulation for IVF, 364 surplus oocytes from 28 patients were vitrified using SN(2). Three hundred two (85.1% +/- 2.9%) of the oocytes survived after warming. Fertilization and cleavage rates were 77.4% +/- 3.5% (168/218) and 94.3% +/- 2.1% (158/168), respectively. Thirteen pregnancies (43.3%) resulted from 30 uterine transfers of 120 embryos with an implantation rate of 14.2% (17/120). There were no differences between the pregnancy rate after vitrification/warming and that obtained from routine noncryopreserved oocytes. CONCLUSION(S): The present report suggests that super-rapid cooling may improve the clinical efficacy of human oocyte vitrification and may be a valuable tool for human assisted reproductive technologies.
Authors: Ho-Joon Lee; Heidi Elmoazzen; Diane Wright; John Biggers; Bo R Rueda; Yun Seok Heo; Mehmet Toner; Thomas L Toth Journal: Reprod Biomed Online Date: 2009-11-27 Impact factor: 3.828
Authors: Bo Yeun Kim; Sook-Young Yoon; Soo Kyoung Cha; Ki Hoon Kwak; Rafael A Fissore; Jan B Parys; Tae Ki Yoon; Dong Ryul Lee Journal: Pflugers Arch Date: 2011-03-23 Impact factor: 3.657
Authors: Min Xu; Erin R West-Farrell; Richard L Stouffer; Lonnie D Shea; Teresa K Woodruff; Mary B Zelinski Journal: Biol Reprod Date: 2009-05-27 Impact factor: 4.285