Literature DB >> 1734929

PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes.

S S Sommer1, A R Groszbach, C D Bottema.   

Abstract

Previous data suggested that PCR amplification of specific alleles (also known as allele-specific amplification and amplification refractory mutation system) is not a general method for rapidly and accurately detecting known single-base changes. Herein we present our experience with the use of PCR amplification of specific alleles to detect 69 polymorphic or mutant alleles. Our results indicate that with proper optimization, all alleles were reliably distinguished.

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Year:  1992        PMID： 1734929

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  33 in total

1.  Jewish and Middle Eastern non-Jewish populations share a common pool of Y-chromosome biallelic haplotypes.

Authors:  M F Hammer; A J Redd; E T Wood; M R Bonner; H Jarjanazi; T Karafet; S Santachiara-Benerecetti; A Oppenheim; M A Jobling; T Jenkins; H Ostrer; B Bonne-Tamir
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-06       Impact factor: 11.205

2.  Single tube genotyping of sickle cell anaemia using PCR-based SNP analysis.

Authors:  C M Waterfall; B D Cobb
Journal:  Nucleic Acids Res       Date:  2001-12-01       Impact factor: 16.971

3.  Molecular haplotyping at high throughput.

Authors:  Jörg Tost; Ole Brandt; Francis Boussicault; David Derbala; Christophe Caloustian; Doris Lechner; Ivo Glynne Gut
Journal:  Nucleic Acids Res       Date:  2002-10-01       Impact factor: 16.971

4.  Detection of MDR1 single nucleotide polymorphisms C3435T and G2677T using real-time polymerase chain reaction: MDR1 single nucleotide polymorphism genotyping assay.

Authors:  Pengfei Song; Shen Li; Bernd Meibohm; A Osama Gaber; Marsha R Honaker; Malak Kotb; Charles R Yates
Journal:  AAPS PharmSci       Date:  2002

5.  The dominance of the herbicide resistance cost in several Arabidopsis thaliana mutant lines.

Authors:  Fabrice Roux; Jacques Gasquez; Xavier Reboud
Journal:  Genetics       Date:  2004-01       Impact factor: 4.562

6.  A mutation in the 14 alpha-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor.

Authors:  C Délye; F Laigret; M F Corio-Costet
Journal:  Appl Environ Microbiol       Date:  1997-08       Impact factor: 4.792

7.  Use of amplification refractory mutation system PCR assay as a simple and effective tool to detect HIV-1 drug resistance mutations.

Authors:  Aubin J Nanfack; Lucy Agyingi; Jean Jacques N Noubiap; Johnson N Ngai; Vittorio Colizzi; Phillipe N Nyambi
Journal:  J Clin Microbiol       Date:  2015-03-18       Impact factor: 5.948

8.  Evidence that adaptation in Drosophila is not limited by mutation at single sites.

Authors:  Talia Karasov; Philipp W Messer; Dmitri A Petrov
Journal:  PLoS Genet       Date:  2010-06-17       Impact factor: 5.917

9.  Fluorescent duplex allele-specific PCR and amplicon melting for rapid homogeneous mtDNA haplogroup H screening and sensitive mixture detection.

Authors:  Harald Niederstätter; Walther Parson
Journal:  PLoS One       Date:  2009-12-18       Impact factor: 3.240

10.  Two new mutations in the porphobilinogen deaminase gene and a screening method using PCR amplification of specific alleles.

Authors:  G Lundin; A Wedell; S Thunell; M Anvret
Journal:  Hum Genet       Date:  1994-01       Impact factor: 4.132
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