Literature DB >> 173425

[Isolation of highly purified ribonuclease from cobra (Naja oxiana) venom].

S K Vasilenko, V C Ryte.   

Abstract

Dialysis, gel-chromatography on Sephadex G-75 (superfine) and chromatography on sulphoethylcellulose give high yield (68 per cent) of 162-fold purified ribonuclease from cobra venom. In ion-exchange chromatography, ribonuclease is eluted in two fractions. The fraction with the highest specific activity has a molecular weight of 15900 and is homogeneous in 15 per cent polyacrilamide gel electrophoresis at pH 8.9. Electrophoresis at pH 4.3 reveals a minor fast component of this fraction which also exhibits a ribonuclease activity. Sulphoethylcellulose chromatography fairly separates cobra venom phosphodiesterase and 5'-nucleotidase eluted as a single fraction in gel chromatography.

Entities:  

Mesh：

Substances：

Year:  1975        PMID： 173425

Source DB:  PubMed          Journal:  Biokhimiia        ISSN： 0320-9725


  32 in total

1.  Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents.

Authors:  M Osswald; B Greuer; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

2.  Two zinc finger proteins from Xenopus laevis bind the same region of 5S RNA but with different nuclease protection patterns.

Authors:  M S Sands; D F Bogenhagen
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

3.  Evidence that genomic and antigenomic RNA self-cleaving elements from hepatitis delta virus have similar secondary structures.

Authors:  S P Rosenstein; M D Been
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

4.  RNase YI* and RNA structure studies.

Authors:  V J Cannistraro; D Kennell
Journal:  Nucleic Acids Res       Date:  1997-04-01       Impact factor: 16.971

5.  Secondary structure of the leader transcript from the Escherichia coli S10 ribosomal protein operon.

Authors:  P Shen; J M Zengel; L Lindahl
Journal:  Nucleic Acids Res       Date:  1988-09-26       Impact factor: 16.971

Review 6.  Probing the structure of RNAs in solution.

Authors:  C Ehresmann; F Baudin; M Mougel; P Romby; J P Ebel; B Ehresmann
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971

7.  Identification of defined sequences in domain V of E. coli 23S rRNA in the 50S subunit accessible for hybridization with complementary oligodeoxyribonucleotides.

Authors:  R T Marconi; W E Hill
Journal:  Nucleic Acids Res       Date:  1988-02-25       Impact factor: 16.971

8.  Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence.

Authors:  C R Woese; L J Magrum; R Gupta; R B Siegel; D A Stahl; J Kop; N Crawford; J Brosius; R Gutell; J J Hogan; H F Noller
Journal:  Nucleic Acids Res       Date:  1980-05-24       Impact factor: 16.971

9.  The secondary structure of oocyte and somatic 5S ribosomal RNAs of the fish Misgurnus fossilis L. from nuclease hydrolyses and chemical modification data.

Authors:  T I Serenkova; A M Mazo; T D Mashkova; I Toots; A Nigul; L L Kisselev
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971

10.  The conformation of chicken, rat and human U1A RNAs in solution.

Authors:  C Branlant; A Krol; J P Ebel; H Gallinaro; E Lazar; M Jacob
Journal:  Nucleic Acids Res       Date:  1981-02-25       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.