"One-shot" velocity microscopy: NMR imaging of motion using a single phase-encoding step.

The use of the pulsed gradient spin-echo sequence in NMR microscopy enables the measurement of molecular translational motion and simultaneous construction of velocity and self-diffusion images, a technique that has been termed dynamic NMR microscopy. In this method the PGSE contrast gradient is stepped in a fourth dimension (q space) and so is inherently inefficient. Provided that one is prepared to sacrifice some of the additional information provided by the multiple PGSE gradient approach, it is possible to construct a velocity image alone by means of a single PGSE phase-encoding step. We illustrate applications of this method in which a signal from the stationary spins is nulled by the use of both gradient phase cycling and a final "z-storage" rf pulse. The limits to velocity resolution are around 10 microns s-1 in free water but can be considerably smaller for molecules with a low self-diffusion coefficient. We demonstrate this method in a study of water capillary flow at 12 microns transverse pixel resolution, extending the velocity range by employing a four-quadrant analysis method. This method is also used to measure vascular transport in a living plant and find a flow rate of around 45 microns s-1.