| Literature DB >> 17339846 |
Breton Hornblower1, Amy Coombs, Richard D Whitaker, Anatoly Kolomeisky, Stephen J Picone, Amit Meller, Mark Akeson.
Abstract
We present a method for rapid measurement of DNA-protein interactions using voltage-driven threading of single DNA molecules through a protein nanopore. Electrical force applied to individual ssDNA-exonuclease I complexes pulls the two molecules apart, while ion current probes the dissociation rate of the complex. Nanopore force spectroscopy (NFS) reveals energy barriers affecting complex dissociation. This method can be applied to other nucleic acid-protein complexes, using protein or solid-state nanopore devices.Mesh：
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Year: 2007 PMID： 17339846 DOI： 10.1038/nmeth1021
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547