Literature DB >> 17336470

Rapid detection of Escherichia coli virulence factor genes using multiplex real-time TaqMan PCR assays.

D M West1, K A Sprigings, C Cassar, P R Wakeley, J Sawyer, R H Davies.   

Abstract

Three multiplex real-time TaqMan PCR assays were developed for the detection of Escherichia coli virulence factor genes in veterinary samples. Target virulence factors chosen were the fimbriae K88 (F4), K99 (F5), F41, F17, F18 and 987p (F6) and the toxins LT, STa and CDT IV. Detection of genes coding GAD were included in each assay as an internal control. These assays allow rapid identification of virulence factor genes using identical cycling conditions on an Mx3000Ptrade mark real-time PCR machine with the capacity to test up to 20 strains for 9 virulence genes in 1h.

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Year:  2007        PMID： 17336470     DOI： 10.1016/j.vetmic.2007.01.026

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  5 in total

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2.  Molecules produced by probiotics prevent enteric colibacillosis in pigs.

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4.  Development of a new TaqMan-based real-time RT-PCR assay for the specific detection of bovine kobuvirus.

Authors:  Yuelin Liu; Libing Liu; Jinfeng Wang; Ting Wang; Yaxin Gao; Xiaoxia Sun; Wanzhe Yuan; Ruiwen Li; Jianchang Wang
Journal:  Front Vet Sci       Date:  2022-08-04

5.  A novel multiplex PCR-electronic microarray assay for rapid and simultaneous detection of bovine respiratory and enteric pathogens.

Authors:  Niroshan Thanthrige-Don; Oliver Lung; Tara Furukawa-Stoffer; Cody Buchanan; Tomy Joseph; Dale L Godson; John Gilleard; Trevor Alexander; Aruna Ambagala
Journal:  J Virol Methods       Date:  2018-08-10       Impact factor: 2.014
  5 in total

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