BACKGROUND: Interleukin-10 (IL-10), an anti-inflammatory cytokine, plays a role in periodontal disease by inhibiting synthesis of proinflammatory cytokines and stimulating protective antibody production. Genetic polymorphisms in the IL-10 gene might be useful as a marker to diagnose susceptibility to periodontitis. Therefore, the aim of this study was to investigate the association between IL-10 gene polymorphisms and severe generalized chronic periodontitis (CP) in a Turkish population. METHODS: Samples of venous blood and DNA were obtained from 75 patients with severe generalized CP and 73 healthy subjects. The IL-10 promoter sequences at positions -597 and -824 were amplified by polymerase chain reaction, and polymorphisms were detected by restriction enzyme cleavage. Genotype and allele frequencies were calculated, and data were analyzed using the chi(2) test. RESULTS: There was a statistically significant difference in frequencies of genotypes (AA/CC + CA: P = 0.00007, odds ratio = 12.37, 95% confidence intervals = 2.74 to 7.77; CC/CA + AA: P = 0.001, odds ratio = 3.05, 95% confidence intervals = 1.47 to 6.33) and alleles (P = 0.0002, odds ratio = 2.61, 95% confidence intervals = 1.52 to 4.51) at position -597 C to A between patients with severe generalized CP and healthy controls, whereas there was no significant difference in genotypes and allele frequencies at position -824 C to T between patients with CP and healthy subjects. CONCLUSION: Within the limitations of sample selection and number, the IL-10 gene polymorphism at position -597 seems to be associated with severe generalized CP.
BACKGROUND:Interleukin-10 (IL-10), an anti-inflammatory cytokine, plays a role in periodontal disease by inhibiting synthesis of proinflammatory cytokines and stimulating protective antibody production. Genetic polymorphisms in the IL-10 gene might be useful as a marker to diagnose susceptibility to periodontitis. Therefore, the aim of this study was to investigate the association between IL-10 gene polymorphisms and severe generalized chronic periodontitis (CP) in a Turkish population. METHODS: Samples of venous blood and DNA were obtained from 75 patients with severe generalized CP and 73 healthy subjects. The IL-10 promoter sequences at positions -597 and -824 were amplified by polymerase chain reaction, and polymorphisms were detected by restriction enzyme cleavage. Genotype and allele frequencies were calculated, and data were analyzed using the chi(2) test. RESULTS: There was a statistically significant difference in frequencies of genotypes (AA/CC + CA: P = 0.00007, odds ratio = 12.37, 95% confidence intervals = 2.74 to 7.77; CC/CA + AA: P = 0.001, odds ratio = 3.05, 95% confidence intervals = 1.47 to 6.33) and alleles (P = 0.0002, odds ratio = 2.61, 95% confidence intervals = 1.52 to 4.51) at position -597 C to A between patients with severe generalized CP and healthy controls, whereas there was no significant difference in genotypes and allele frequencies at position -824 C to T between patients with CP and healthy subjects. CONCLUSION: Within the limitations of sample selection and number, the IL-10 gene polymorphism at position -597 seems to be associated with severe generalized CP.
Authors: Sam S Oh; Shen-Chih Chang; Lin Cai; Carlos Cordon-Cardo; Bao-Guo Ding; Sander Greenland; Na He; Qingwu Jiang; Leeka Kheifets; Anh Le; Yuan-Chin Amy Lee; Simin Liu; Ming-Lan Lu; Jenny T Mao; Hal Morgenstern; Li-Na Mu; Allan Pantuck; Jeanette C Papp; Sungshim Lani Park; Jian Yu Rao; Victor E Reuter; Donald P Tashkin; Hua Wang; Nai-Chieh Y You; Shun-Zhang Yu; Jin-Kou Zhao; Arie Belldegrun; Zuo-Feng Zhang Journal: Int J Cancer Date: 2010-11-01 Impact factor: 7.396
Authors: Ariadne Letra; Renato M Silva; Ryan J Rylands; Elcia M Silveira; Ana P de Souza; Steven K Wendell; Gustavo P Garlet; Alexandre R Vieira Journal: J Clin Periodontol Date: 2012-06-03 Impact factor: 8.728
Authors: Wings T Y Loo; Lan-jun Bai; Chang-bin Fan; Yuan Yue; Yi-ding Dou; Min Wang; Hao Liang; Mary N B Cheung; Louis W C Chow; Jin-le Li; Ye Tian; Liu Qing Journal: J Transl Med Date: 2012-09-19 Impact factor: 5.531