PURPOSE: (99m)Tc-UBI 29-41 (UBI), an antimicrobial peptide, specifically targets bacteria. We tested the ability of UBI to discriminate between infected and uninfected prosthetic joints using a rabbit model previously validated. METHODS: Left knee arthroplasty was performed on 20 New Zealand rabbits, then 10(7) cfu of S. aureus (n = 12) or sterile saline (n = 8) was injected into the joint. On days 9 and 20 after surgery, planar UBI scintigraphy was performed in six infected and four uninfected rabbits, 1 h and 4 h p.i. (150 MBq), on a gamma camera. Operated-to-normal knee activity ratio (ONKR) was calculated on each scintigram. Then, after sacrifice, tissue samples of both knees were counted in a gamma counter. RESULTS: One rabbit injected with sterile saline had cutaneous infection at sacrifice and was excluded from analysis. ONKR was higher in infected than in uninfected animals 4 h p.i. 20 days after surgery: 1.75 +/- 0.48 vs 1.13 +/- 0.11, p = 0.04. From 1 h to 4 h p.i., ONKR increased in 9/12 infected and 0/7 uninfected animals. According to UBI uptake intensity and kinetics, scintigraphy was truly positive in all infected cases on day 9 and in four of six infected cases on day 20. It was truly negative in two of three sterile inflamed prosthetic knees on day 9, and in all cases on day 20. Biodistribution studies revealed increased UBI uptake in periprosthetic tissues in all animals 9 days after surgery, and only in infected animals on day 20. CONCLUSION: In this experimental study, (99m)Tc-UBI 29-41 scintigraphy permitted the early detection of acute prosthetic joint infection, and exclusion of infection in chronic sterile prosthetic joint inflammation.
PURPOSE: (99m)Tc-UBI 29-41 (UBI), an antimicrobial peptide, specifically targets bacteria. We tested the ability of UBI to discriminate between infected and uninfected prosthetic joints using a rabbit model previously validated. METHODS: Left knee arthroplasty was performed on 20 New Zealand rabbits, then 10(7) cfu of S. aureus (n = 12) or sterile saline (n = 8) was injected into the joint. On days 9 and 20 after surgery, planar UBI scintigraphy was performed in six infected and four uninfected rabbits, 1 h and 4 h p.i. (150 MBq), on a gamma camera. Operated-to-normal knee activity ratio (ONKR) was calculated on each scintigram. Then, after sacrifice, tissue samples of both knees were counted in a gamma counter. RESULTS: One rabbit injected with sterile saline had cutaneous infection at sacrifice and was excluded from analysis. ONKR was higher in infected than in uninfected animals 4 h p.i. 20 days after surgery: 1.75 +/- 0.48 vs 1.13 +/- 0.11, p = 0.04. From 1 h to 4 h p.i., ONKR increased in 9/12 infected and 0/7 uninfected animals. According to UBI uptake intensity and kinetics, scintigraphy was truly positive in all infected cases on day 9 and in four of six infected cases on day 20. It was truly negative in two of three sterile inflamed prosthetic knees on day 9, and in all cases on day 20. Biodistribution studies revealed increased UBI uptake in periprosthetic tissues in all animals 9 days after surgery, and only in infected animals on day 20. CONCLUSION: In this experimental study, (99m)Tc-UBI 29-41 scintigraphy permitted the early detection of acute prosthetic joint infection, and exclusion of infection in chronic sterile prosthetic joint inflammation.
Authors: Benedicte Vanquickenborne; Alex Maes; Johan Nuyts; Frank Van Acker; Jos Stuyck; Michiel Mulier; Alfons Verbruggen; Luc Mortelmans Journal: Eur J Nucl Med Mol Imaging Date: 2003-03-04 Impact factor: 9.236
Authors: Louis Bernard; Pierre Hoffmeyer; Mathieu Assal; Pierre Vaudaux; Jacques Schrenzel; Daniel Lew Journal: J Antimicrob Chemother Date: 2003-12-19 Impact factor: 5.790
Authors: A C Crémieux; A S Mghir; R Bleton; M Manteau; N Belmatoug; L Massias; L Garry; N Sales; B Mazière; C Carbon Journal: Antimicrob Agents Chemother Date: 1996-09 Impact factor: 5.191
Authors: Mick M Welling; Sandra Mongera; Antonella Lupetti; Henia S Balter; Valeria Bonetto; Ulderico Mazzi; Ernest K J Pauwels; Peter H Nibbering Journal: Nucl Med Biol Date: 2002-05 Impact factor: 2.408
Authors: Peter H Nibbering; Mick M Welling; Akke Paulusma-Annema; Carlo P J M Brouwer; Antonella Lupetti; Ernest K J Pauwels Journal: J Nucl Med Date: 2004-02 Impact factor: 10.057
Authors: Filip Gemmel; Hans Van den Wyngaert; Charito Love; M M Welling; Paul Gemmel; Christopher J Palestro Journal: Eur J Nucl Med Mol Imaging Date: 2012-02-24 Impact factor: 9.236
Authors: Stephen D Zoller; Howard Y Park; Tove Olafsen; Charles Zamilpa; Zachary Dc Burke; Gideon Blumstein; William L Sheppard; Christopher D Hamad; Kellyn R Hori; Jen-Chieh Tseng; Julie Czupryna; Craig McMannus; Jason T Lee; Mafalda Bispo; Francisco Romero Pastrana; Elisa Jm Raineri; Jeffery F Miller; Lloyd S Miller; Jan Maarten van Dijl; Kevin P Francis; Nicholas M Bernthal Journal: JCI Insight Date: 2019-02-07