Literature DB >> 17333464

Expression, purification, and characterization of recombinant Chinese shrimp crustin-like protein (CruFc) in Pichia pastoris.

Jiquan Zhang1, Fuhua Li, Zaizhao Wang, Jianhai Xiang.   

Abstract

A crustin-like protein (CruFc) from Fenneropenaeus chinensis was expressed in Pichia pastoris and then purified to electrophoretic homogeneity on a Sephacryl S-100 column with a band corresponding to the expected one (13 kDa) shown by 15% SDS-PAGE. Western blot indicated that the rCruFc specifically reacted with polyclonal rabbit anti-Fenneropenaeus chinensis CruFc. Production in a 5 l bioreactor gave 237 mg rCruFc/l. Antimicrobial assay revealed that 4 microM rCruFc inhibited growth of Staphylococcus aureus.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17333464     DOI： 10.1007/s10529-007-9317-6

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  4 in total

1.  Functional Characterization of a c-type Lysozyme from Indian Shrimp Fenneropenaeus indicus.

Authors:  Viswanathan Karthik; Vijayan Kamalakannan; Ancy Thomas; Naduvilamuriparambu Saidumuhammed Sudheer; Issac S Bright Singh; Rangarajan Badri Narayanan
Journal:  Probiotics Antimicrob Proteins       Date:  2014-06       Impact factor: 4.609

2.  Molecular cloning and characterization of crustin from mud crab Scylla paramamosain.

Authors:  Chanprapa Imjongjirak; Piti Amparyup; Anchalee Tassanakajon; Siriporn Sittipraneed
Journal:  Mol Biol Rep       Date:  2008-04-19       Impact factor: 2.316

3.  Identification and molecular characterization of a C-type lectin-like protein from Chinese shrimp (Fenneropenaeus chinensis).

Authors:  Xiaofang Lai; Jie Kong; Qingyin Wang; Weiji Wang; Xianhong Meng
Journal:  Mol Biol Rep       Date:  2012-11-29       Impact factor: 2.316

4.  A New Crustin Gene Homolog SpCrus8 Identified in Scylla paramamosain Exerting In Vivo Protection Through Opsonization and Immunomodulation.

Authors:  Manyu Jiang; Roushi Chen; Fangyi Chen; Xuewu Zhu; Ke-Jian Wang
Journal:  Front Immunol       Date:  2022-07-08       Impact factor: 8.786
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.