Literature DB >> 17332630

Extraction of plant RNA.

Elspeth MacRae1.   

Abstract

Optimal sampling procedures for sampling plant tissue for RNA extractions are outlined in this chapter. To extract RNA, kits supplied from biotechnology companies are appropriate, but some procedures will not work with particular plant tissues. Two alternative methods are supplied for troublesome material. Basic methods to check RNA quantity and, more particularly, RNA quality before use are supplied.

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Year:  2007        PMID: 17332630     DOI: 10.1385/1-59745-229-7:15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  9 in total

1.  Identification of differentially expressed genes under heat stress conditions in rice (Oryza sativa L.).

Authors:  Mustaq Mohammed S Wahab; Srividhya Akkareddy; P Shanthi; P Latha
Journal:  Mol Biol Rep       Date:  2020-02-17       Impact factor: 2.316

2.  Extraction of high quality RNA from polysaccharide matrices using cetyltrimethylammonium bromide.

Authors:  Limin Wang; Jan P Stegemann
Journal:  Biomaterials       Date:  2009-12-03       Impact factor: 12.479

3.  Chitosanase-based method for RNA isolation from cells transfected with chitosan/siRNA nanocomplexes for real-time RT-PCR in gene silencing.

Authors:  Mohamad Alameh; Myriam Jean; Diogo Dejesus; Michael D Buschmann; Abderrazzak Merzouki
Journal:  Int J Nanomedicine       Date:  2010-08-09

4.  Protocol: high throughput silica-based purification of RNA from Arabidopsis seedlings in a 96-well format.

Authors:  Eliane Salvo-Chirnside; Steven Kane; Lorraine E Kerr
Journal:  Plant Methods       Date:  2011-12-02       Impact factor: 4.993

5.  Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing.

Authors:  Yang Yang; Weixing Shan
Journal:  Bio Protoc       Date:  2021-09-20

6.  A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics.

Authors:  Huogen Xiao; Won-Sik Kim; Baozhong Meng
Journal:  Virol J       Date:  2015-10-20       Impact factor: 4.099

7.  The Impact of Heat Stress on Morpho-Physiological Response and Expression of Specific Genes in the Heat Stress-Responsive Transcriptional Regulatory Network in Brassica oleracea.

Authors:  Mahdi Moradpour; Siti Nor Akmar Abdullah; Parameswari Namasivayam
Journal:  Plants (Basel)       Date:  2021-05-26

8.  Methods of high integrity RNA extraction from cell/agarose construct.

Authors:  Takahiro Ogura; Akihiro Tsuchiya; Tom Minas; Shuichi Mizuno
Journal:  BMC Res Notes       Date:  2015-11-04

9.  AM-Induced Alteration in the Expression of Genes, Encoding Phosphorus Transporters and Enzymes of Carbohydrate Metabolism in Medicago lupulina.

Authors:  Andrey Yurkov; Alexey Kryukov; Anastasia Gorbunova; Andrey Sherbakov; Ksenia Dobryakova; Yulia Mikhaylova; Alexey Afonin; Maria Shishova
Journal:  Plants (Basel)       Date:  2020-04-10
  9 in total

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