| Literature DB >> 17330739 |
Audun H Nerland1, Cecilie Skaar, Tove Boge Eriksen, Hogne Bleie.
Abstract
We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 x 10(4) TCID50 (50% tissue culture infectious dose) ml(-1). Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 x 10(7) virus particles ml(-1) seawater.Entities:
Mesh:
Year: 2007 PMID: 17330739 DOI: 10.3354/dao073201
Source DB: PubMed Journal: Dis Aquat Organ ISSN: 0177-5103 Impact factor: 1.802