Literature DB >> 17325365

Development and characterization of an infectious cDNA clone of the virulent Bucyrus strain of Equine arteritis virus.

Udeni B R Balasuriya1, Eric J Snijder, Hans W Heidner, Jianqiang Zhang, Jessika C Zevenhoven-Dobbe, Josh D Boone, William H McCollum, Peter J Timoney, N James MacLachlan.   

Abstract

Strains of Equine arteritis virus (EAV) differ in the severity of the disease that they induce in horses. Infectious cDNA clones are potentially useful for identification of genetic determinants of EAV virulence; to date, two clones have been derived from a cell culture-adapted variant of the original (Bucyrus) isolate of EAV, and it has previously been shown that recombinant virus derived from one of these (rEAV030) is attenuated in horses. A complete cDNA copy of the genome of the virulent Bucyrus strain of EAV has now been assembled into a plasmid vector. In contrast to rEAV030, recombinant progeny virus derived from this clone caused severe disease in horses, characterized by pyrexia, oedema, leukopenia, high-titre viraemia and substantial nasal shedding of virus. The availability of infectious cDNA clones that produce recombinant viruses of different virulence to horses will facilitate characterization of the virulence determinants of EAV through reverse genetics.

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Year:  2007        PMID： 17325365     DOI： 10.1099/vir.0.82415-0

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  20 in total

1.  Equine Arteritis Virus Has Specific Tropism for Stromal Cells and CD8+ T and CD21+ B Lymphocytes but Not for Glandular Epithelium at the Primary Site of Persistent Infection in the Stallion Reproductive Tract.

Authors:  Mariano Carossino; Alan T Loynachan; Igor F Canisso; R Frank Cook; Juliana R Campos; Bora Nam; Yun Young Go; Edward L Squires; Mats H T Troedsson; Thomas Swerczek; Fabio Del Piero; Ernest Bailey; Peter J Timoney; Udeni B R Balasuriya
Journal:  J Virol       Date:  2017-06-09       Impact factor: 5.103

2.  Development and characterization of an infectious cDNA clone of the modified live virus vaccine strain of equine arteritis virus.

Authors:  Jianqiang Zhang; Yun Young Go; Chengjin M Huang; Barry J Meade; Zhengchun Lu; Eric J Snijder; Peter J Timoney; Udeni B R Balasuriya
Journal:  Clin Vaccine Immunol       Date:  2012-06-27

3.  Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes.

Authors:  Yun Young Go; Jianqiang Zhang; Peter J Timoney; R Frank Cook; David W Horohov; Udeni B R Balasuriya
Journal:  J Virol       Date:  2010-03-10       Impact factor: 5.103

4.  Equine Arteritis Virus Elicits a Mucosal Antibody Response in the Reproductive Tract of Persistently Infected Stallions.

Authors:  Mariano Carossino; Bettina Wagner; Alan T Loynachan; R Frank Cook; Igor F Canisso; Lakshman Chelvarajan; Casey L Edwards; Bora Nam; John F Timoney; Peter J Timoney; Udeni B R Balasuriya
Journal:  Clin Vaccine Immunol       Date:  2017-10-05

5.  Downregulation of MicroRNA eca-mir-128 in Seminal Exosomes and Enhanced Expression of CXCL16 in the Stallion Reproductive Tract Are Associated with Long-Term Persistence of Equine Arteritis Virus.

Authors:  Mariano Carossino; Pouya Dini; Theodore S Kalbfleisch; Alan T Loynachan; Igor F Canisso; Kathleen M Shuck; Peter J Timoney; R Frank Cook; Udeni B R Balasuriya
Journal:  J Virol       Date:  2018-04-13       Impact factor: 5.103

6.  Characterization of equine humoral antibody response to the nonstructural proteins of equine arteritis virus.

Authors:  Yun Young Go; Eric J Snijder; Peter J Timoney; Udeni B R Balasuriya
Journal:  Clin Vaccine Immunol       Date:  2010-12-08

7.  Persistent equine arteritis virus infection in HeLa cells.

Authors:  Jianqiang Zhang; Peter J Timoney; N James MacLachlan; William H McCollum; Udeni B R Balasuriya
Journal:  J Virol       Date:  2008-06-25       Impact factor: 5.103

8.  Each of the eight simian hemorrhagic fever virus minor structural proteins is functionally important.

Authors:  Heather A Vatter; Han Di; Eric F Donaldson; Ralph S Baric; Margo A Brinton
Journal:  Virology       Date:  2014-07-16       Impact factor: 3.616

9.  Development of a fluorescent-microsphere immunoassay for detection of antibodies specific to equine arteritis virus and comparison with the virus neutralization test.

Authors:  Yun Young Go; Susan J Wong; Adam J Branscum; Valerie L Demarest; Kathleen M Shuck; Mary L Vickers; Jianqiang Zhang; William H McCollum; Peter J Timoney; Udeni B R Balasuriya
Journal:  Clin Vaccine Immunol       Date:  2007-11-21

10.  Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor.

Authors:  Sanjay Sarkar; Lakshman Chelvarajan; Yun Young Go; Frank Cook; Sergey Artiushin; Shankar Mondal; Kelsi Anderson; John Eberth; Peter J Timoney; Theodore S Kalbfleisch; Ernest Bailey; Udeni B R Balasuriya
Journal:  J Virol       Date:  2016-01-13       Impact factor: 5.103
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