OBJECTIVE: Cytokines are inflammatory mediators implicated in abdominal aortic aneurysm (AAA) pathogenesis. The cytokine expression profile of the AAA is poorly defined and has focused on the expression of pro-inflammatory cytokines, at the expense of chemokines and growth factors. This study aims to investigate the cytokine expression profile of the established AAA wall. METHODS: Cytokine protein expression was measured in homogenized human aortic tissue (10 AAAs and 9 nonaneurysmal controls) using a 42-cytokine antibody-based protein array. Data were quantified using densitometric analysis and statistically analyzed using a Mann-Whitney U test. RESULTS: A significant difference in cytokine expression between AAA and control samples was found in 15 of 42 cytokines. Several pro-inflammatory cytokines were upregulated within the AAA compared with the control: interleukin (IL)-6 (P = .001), IL-1alpha (P = .001), IL-1beta (P < .001), tumor necrosis factor (TNF)-alpha (P = .002), TNF-beta (P = .002), and oncostatin M (P = .007). The anti-inflammatory cytokine IL-10 was also upregulated (P = .002). Members of the chemokine family were also highly expressed within AAA samples: IL-8 (P = .001), epithelial neutrophil-activating peptide-78 (ENA-78; P = .006), growth related oncogene (GRO; P < .001), monocyte chemoattractant protein (MCP)-1 (P = .003), MCP-2 (P < .001), and regulated upon activation, normal T-cell expressed and secreted (RANTES; P = .001). Of the growth factors examined, granulocyte colony-stimulating factor (GCSF; P = .003) and macrophage colony-stimulating factor (MCSF; P = .004) were significantly higher in the AAA. CONCLUSIONS: The established AAA is characterized by a distinct cytokine profile consisting of pro-inflammatory cytokines, chemokines, and specific growth factors. This suggests that these cytokines may contribute to pathologic changes within the established, preruptured aneurysm.
OBJECTIVE: Cytokines are inflammatory mediators implicated in abdominal aortic aneurysm (AAA) pathogenesis. The cytokine expression profile of the AAA is poorly defined and has focused on the expression of pro-inflammatory cytokines, at the expense of chemokines and growth factors. This study aims to investigate the cytokine expression profile of the established AAA wall. METHODS: Cytokine protein expression was measured in homogenized human aortic tissue (10 AAAs and 9 nonaneurysmal controls) using a 42-cytokine antibody-based protein array. Data were quantified using densitometric analysis and statistically analyzed using a Mann-Whitney U test. RESULTS: A significant difference in cytokine expression between AAA and control samples was found in 15 of 42 cytokines. Several pro-inflammatory cytokines were upregulated within the AAA compared with the control: interleukin (IL)-6 (P = .001), IL-1alpha (P = .001), IL-1beta (P < .001), tumor necrosis factor (TNF)-alpha (P = .002), TNF-beta (P = .002), and oncostatin M (P = .007). The anti-inflammatory cytokine IL-10 was also upregulated (P = .002). Members of the chemokine family were also highly expressed within AAA samples: IL-8 (P = .001), epithelial neutrophil-activating peptide-78 (ENA-78; P = .006), growth related oncogene (GRO; P < .001), monocyte chemoattractant protein (MCP)-1 (P = .003), MCP-2 (P < .001), and regulated upon activation, normal T-cell expressed and secreted (RANTES; P = .001). Of the growth factors examined, granulocyte colony-stimulating factor (GCSF; P = .003) and macrophage colony-stimulating factor (MCSF; P = .004) were significantly higher in the AAA. CONCLUSIONS: The established AAA is characterized by a distinct cytokine profile consisting of pro-inflammatory cytokines, chemokines, and specific growth factors. This suggests that these cytokines may contribute to pathologic changes within the established, preruptured aneurysm.
Authors: Antonio Di Gennaro; Dick Wågsäter; Mikko I Mäyränpää; Anders Gabrielsen; Jesper Swedenborg; Anders Hamsten; Bengt Samuelsson; Per Eriksson; Jesper Z Haeggström Journal: Proc Natl Acad Sci U S A Date: 2010-11-15 Impact factor: 11.205
Authors: Annet Kirabo; Sergey Ryzhov; Manisha Gupte; Seng Sengsayadeth; Richard J Gumina; Douglas B Sawyer; Cristi L Galindo Journal: J Mol Cell Cardiol Date: 2017-03-03 Impact factor: 5.000
Authors: Jonathan Golledge; Paula Clancy; Corey Moran; Erik Biros; Catherine Rush; Philip Walker; Paul Norman Journal: Am J Pathol Date: 2010-03-26 Impact factor: 4.307
Authors: P Escudero; A Navarro; C Ferrando; E Furio; H Gonzalez-Navarro; M Juez; M J Sanz; L Piqueras Journal: Br J Pharmacol Date: 2015-03-26 Impact factor: 8.739
Authors: Mengyang Liao; Cong-Lin Liu; Bing-Jie Lv; Jin-Ying Zhang; Longxian Cheng; Xiang Cheng; Jes S Lindholt; Lars M Rasmussen; Guo-Ping Shi Journal: Ann Med Date: 2015-04-09 Impact factor: 4.709
Authors: Hui-fang Zhou; Huimin Yan; Judy L Cannon; Luke E Springer; Jonathan M Green; Christine T N Pham Journal: J Immunol Date: 2013-04-12 Impact factor: 5.422