Lixing Yan1, Junjie Wu, Faming Zhu, Xiaozhen Hong, Xianguo Xu. 1. Institute of Transfusion Medicine, Blood Center of Zhejiang Province, and Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, China. ylx@zjb.org.cn
Abstract
BACKGROUND: Most studies of the molecular basis of D variants have been conducted in Caucasian and African populations. There are limited data on the molecular basis for D variants in Chinese populations. STUDY DESIGN AND METHODS: With a blended monoclonal anti-D and a sequential slide and tube typing protocol, red blood cells from greater than 99 percent of Han Chinese were tested for the D antigen. Samples that agglutinated weakly by tube method or that reacted only by indirect antiglobulin test (IAT) were classified as D variants. The D variant was tested by an RHD polymerase chain reaction with sequence specific primers and by gene sequencing to distinguish and characterize weak D and partial D alleles. RESULTS: Of 305,572 samples from individual donations (305,475 [99.97%] were ethnic Han and 97 [0.032%] were ethnic minorities), 304,134 (99.53%) typed as D+. Five (0.0016%) typed as D variants (weak agglutination by tube). By IAT an additional 32 (0.0105%) typed as D variants and 1401 (0.46%) typed as D-. Weak D type 15 and RHD(K409K) alleles represented 72.7 percent of all weak D phenotypes. All partial D phenotypes were DVI Type 3 or DV. Three new weak D alleles carrying 594A>T and 602C>G (weak D Type 51), 92T>C (weak D type 52), and 740T>G (weak D type 53) mutations, respectively, were identified. CONCLUSION: There are significant differences in the frequencies and molecular characteristics of D variants among indigenous Chinese populations, compared to Caucasian and African populations, which must be considered when developing clinical practices related to D variant blood donors, transfusion recipients, or obstetrical patients.
BACKGROUND: Most studies of the molecular basis of D variants have been conducted in Caucasian and African populations. There are limited data on the molecular basis for D variants in Chinese populations. STUDY DESIGN AND METHODS: With a blended monoclonal anti-D and a sequential slide and tube typing protocol, red blood cells from greater than 99 percent of Han Chinese were tested for the D antigen. Samples that agglutinated weakly by tube method or that reacted only by indirect antiglobulin test (IAT) were classified as D variants. The D variant was tested by an RHD polymerase chain reaction with sequence specific primers and by gene sequencing to distinguish and characterize weak D and partial D alleles. RESULTS: Of 305,572 samples from individual donations (305,475 [99.97%] were ethnic Han and 97 [0.032%] were ethnic minorities), 304,134 (99.53%) typed as D+. Five (0.0016%) typed as D variants (weak agglutination by tube). By IAT an additional 32 (0.0105%) typed as D variants and 1401 (0.46%) typed as D-. Weak D type 15 and RHD(K409K) alleles represented 72.7 percent of all weak D phenotypes. All partial D phenotypes were DVI Type 3 or DV. Three new weak D alleles carrying 594A>T and 602C>G (weak D Type 51), 92T>C (weak D type 52), and 740T>G (weak D type 53) mutations, respectively, were identified. CONCLUSION: There are significant differences in the frequencies and molecular characteristics of D variants among indigenous Chinese populations, compared to Caucasian and African populations, which must be considered when developing clinical practices related to D variant blood donors, transfusion recipients, or obstetricalpatients.
Authors: S Gerald Sandler; Willy A Flegel; Connie M Westhoff; Gregory A Denomme; Meghan Delaney; Margaret A Keller; Susan T Johnson; Louis Katz; John T Queenan; Ralph R Vassallo; Clayton D Simon Journal: Transfusion Date: 2014-12-01 Impact factor: 3.157
Authors: Seema Kacker; Ralph Vassallo; Margaret A Keller; Connie M Westhoff; Kevin D Frick; S Gerald Sandler; Aaron A R Tobian Journal: Transfusion Date: 2015-03-21 Impact factor: 3.157