Literature DB >> 17317144

Chlamydomonas reinhardtii chloroplasts as protein factories.

Stephen P Mayfield1, Andrea L Manuell, Stephen Chen, Joann Wu, Miller Tran, David Siefker, Machiko Muto, Julia Marin-Navarro.   

Abstract

Protein-based therapeutics are the fastest growing sector of drug development, mainly because of the high sensitivity and specificity of these molecules. Their high specificity leads to few side effects and excellent success rates in drug development. However, the inherent complexity of these molecules restricts their synthesis to living cells, making recombinant proteins expensive to produce. In addition to therapeutic uses, recombinant proteins also have a variety of industrial applications and are important research reagents. Eukaryotic algae offer the potential to produce high yields of recombinant proteins more rapidly and at much lower cost than traditional cell culture. Additionally, transgenic algae can be grown in complete containment, reducing any risk of environmental contamination. This system might also be used for the oral delivery of therapeutic proteins, as green algae are edible and do not contain endotoxins or human viral or prion contaminants.

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Year:  2007        PMID: 17317144     DOI: 10.1016/j.copbio.2007.02.001

Source DB:  PubMed          Journal:  Curr Opin Biotechnol        ISSN: 0958-1669            Impact factor:   9.740


  44 in total

1.  The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics.

Authors:  Beth A Rasala; Stephen P Mayfield
Journal:  Bioeng Bugs       Date:  2011 Jan-Feb

2.  Biofuels from algae: challenges and potential.

Authors:  Michael Hannon; Javier Gimpel; Miller Tran; Beth Rasala; Stephen Mayfield
Journal:  Biofuels       Date:  2010-09       Impact factor: 2.956

3.  Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects.

Authors:  Yangmin Gong; Hanhua Hu; Yuan Gao; Xudong Xu; Hong Gao
Journal:  J Ind Microbiol Biotechnol       Date:  2011-09-01       Impact factor: 3.346

Review 4.  Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory.

Authors:  Yong Min Kwon; Kyung Woo Kim; Tae-Young Choi; Sun Young Kim; Jaoon Young Hwan Kim
Journal:  World J Microbiol Biotechnol       Date:  2018-11-26       Impact factor: 3.312

5.  A molecular method for the delivery of small molecules and proteins across the cell wall of algae using molecular transporters.

Authors:  Joel M Hyman; Erika I Geihe; Brian M Trantow; Bahram Parvin; Paul A Wender
Journal:  Proc Natl Acad Sci U S A       Date:  2012-07-30       Impact factor: 11.205

6.  Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene.

Authors:  Henning Kirst; Jose Gines García-Cerdán; Andreas Zurbriggen; Anastasios Melis
Journal:  Plant Physiol       Date:  2011-11-23       Impact factor: 8.340

7.  Natural variants of photosystem II subunit D1 tune photochemical fitness to solar intensity.

Authors:  David J Vinyard; Javier Gimpel; Gennady M Ananyev; Mario A Cornejo; Susan S Golden; Stephen P Mayfield; G Charles Dismukes
Journal:  J Biol Chem       Date:  2012-12-27       Impact factor: 5.157

8.  Production of unique immunotoxin cancer therapeutics in algal chloroplasts.

Authors:  Miller Tran; Christina Van; Daniel J Barrera; Pär L Pettersson; Carlos D Peinado; Jack Bui; Stephen P Mayfield
Journal:  Proc Natl Acad Sci U S A       Date:  2012-12-10       Impact factor: 11.205

9.  Truncated photosystem chlorophyll antenna size in the green microalga Chlamydomonas reinhardtii upon deletion of the TLA3-CpSRP43 gene.

Authors:  Henning Kirst; Jose Gines Garcia-Cerdan; Andreas Zurbriggen; Thilo Ruehle; Anastasios Melis
Journal:  Plant Physiol       Date:  2012-10-05       Impact factor: 8.340

10.  An efficient protocol for the Agrobacterium-mediated genetic transformation of microalga Chlamydomonas reinhardtii.

Authors:  P T Pratheesh; M Vineetha; G Muraleedhara Kurup
Journal:  Mol Biotechnol       Date:  2014-06       Impact factor: 2.695

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