| Literature DB >> 17316678 |
Phillip Scheinberg1, Jan J Melenhorst, Brenna J Hill, Keyvan Keyvanfar, A John Barrett, David A Price, Daniel C Douek.
Abstract
The characterization of the T-cell receptor (TCR) repertoire of CD4+ regulatory T cells (T(R)) has been limited due to the RNA degradation that results following permeabilization and fixation as routinely used for intracellular staining of Foxp3. In the present study the clonal composition of human umbilical cord blood (UCB) and adult peripheral blood mononuclear cell (PBMC) CD4+ T(R) and non-T(R) was characterized by a DNA-based multiplex PCR which allowed for the consistent clonotypic characterization of cells that have undergone fixation and permeabilization. To validate this method, CD8+ T cells from two HLA A()0201 individuals were sorted and compared clonotypically based upon their ability either to secrete interferon-gamma in response to a CMV pp65 epitope or to bind to the corresponding pMHC I tetramer. Clonotypes shared between the CD4+CD25+Foxp3+ and CD4+CD25+Foxp3- subsets were observed in all 3 UCB and in one adult PBMCs, suggesting that naïve and memory CD4+ T(R) can share the same clonotypes as CD4+ non-T(R) in humans.Entities:
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Year: 2007 PMID： 17316678 PMCID： PMC4402232 DOI： 10.1016/j.jim.2007.01.005
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303