Literature DB >> 17316660

Mixer-settler counter-current chromatography with a barricaded spiral disk assembly with glass beads.

Yoichiro Ito1, Lin Qi, Jimmie Powell, Frank Sharpnack, Howard Metger, James Yost, Xue-Li Cao, Yin-Mao Dong, Liang-Sheng Huo, Xiao-Ping Zhu, Ting Li.   

Abstract

A novel spiral disk is designed by placing barricades at 6 mm intervals in the middle of the spiral channel to divide the channel into multiple sections. Glass beads are placed in every other section so that the planetary motion produces repetitive mixing and settling of polymer phase systems. Performance of this mixer-settler spiral disk assembly was examined for separation of lysozyme and myoglobin with a polymer phase system. The best results were obtained with a spiral disk equipped with barricades with openings ranging from 1.2 to 0.4 mm on each side at a high revolution speed up to 1200 rpm.

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Year:  2007        PMID: 17316660     DOI: 10.1016/j.chroma.2006.11.078

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN: 0021-9673            Impact factor:   4.759


  8 in total

1.  Spiral Tube Assembly for High-Speed Countercurrent Chromatography: Choice of Elution Modes for Four Typical Two-Phase Solvent Systems.

Authors:  Y Ito; R Clary; J Powell; M Knight; T M Finn
Journal:  J Liq Chromatogr Relat Technol       Date:  2008-01-01       Impact factor: 1.312

Review 2.  Spiral countercurrent chromatography.

Authors:  Yoichiro Ito; Martha Knight; Thomas M Finn
Journal:  J Chromatogr Sci       Date:  2013-08       Impact factor: 1.618

3.  A simple tool for tubing modification to improve spiral high-speed counter-current chromatography for protein purification.

Authors:  Yoichiro Ito; Xiaofeng Ma; Robert Clary
Journal:  Curr Chromatogr       Date:  2016

4.  Design of a coil satellite centrifuge and its performance on counter-current chromatographic separation of 4-methylumbelliferyl sugar derivatives with polar organic-aqueous two-phase solvent systems.

Authors:  Kazufusa Shinomiya; Koji Tokura; Emiru Kimura; Midori Takai; Naoki Harikai; Kazunori Yoshida; Kazuhiro Yanagidaira; Yoichiro Ito
Journal:  J Chromatogr A       Date:  2015-03-12       Impact factor: 4.759

5.  Spiral column configuration for protein separation by high-speed countercurrent chromatography.

Authors:  Yoichiro Ito
Journal:  Chem Eng Process       Date:  2010-07       Impact factor: 4.237

6.  Development and evaluation of a spiral tube column for counter-current chromatography.

Authors:  Xueli Cao; Hairun Pei; Liangsheng Huo; Guanghui Hu; Yoichiro Ito
Journal:  J Sep Sci       Date:  2011-09-05       Impact factor: 3.645

7.  Improved spiral tube assembly for high-speed counter-current chromatography.

Authors:  Y Ito; R Clary; J Powell; M Knight; T M Finn
Journal:  J Chromatogr A       Date:  2008-11-13       Impact factor: 4.759

8.  Spiral Countercurrent Chromatography Enrichment, Characterization, and Assays of Carbon Nanotube Chiralities for Use in Biosensors.

Authors:  Steingrimur Stefansson; Rodrigo Lazo-Portugal; Saeyoung Ahn; Martha Knight
Journal:  ACS Omega       Date:  2017-03-24
  8 in total

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