Literature DB >> 1731640

Purification and properties of versiconal cyclase from Aspergillus parasiticus.

B K Lin1, J A Anderson.   

Abstract

Versiconal cyclase catalyzes the dehydration of versiconal to versicolorin B or versicolorin C [versicolorin B(C)]. The enzyme was purified from mycelia of Aspergillus parasiticus by DEAE-cellulose, hydroxylapatite, and Mono Q column chromatography. The protein contains two identical subunits of molecular weight 72,000 per molecule of native protein. The pI of the enzyme is 3.95. The pH activity curve had a broad maximum with a peak at 5.5. The Km and Vmax for versiconal at 30 degrees C and pH 6.0 are 3.1 microM and 0.15 mumol min-1mg-1, respectively. Most of the formation of versicolorin B(C) in the cell is attributed to the action of versiconal cyclase.

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Year:  1992        PMID： 1731640     DOI： 10.1016/0003-9861(92)90366-5

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  25 in total

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Authors:  Jiujiang Yu; Perng-Kuang Chang; Kenneth C Ehrlich; Jeffrey W Cary; Deepak Bhatnagar; Thomas E Cleveland; Gary A Payne; John E Linz; Charles P Woloshuk; Joan W Bennett
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792

2.  Purification and characterization of two versiconal hemiacetal acetate reductases involved in aflatoxin biosynthesis.

Authors:  K Matsushima; Y Ando; T Hamasaki; K Yabe
Journal:  Appl Environ Microbiol       Date:  1994-07       Impact factor: 4.792

3.  Purification and Characterization of O-Methyltransferase I Involved in Conversion of Demethylsterigmatocystin to Sterigmatocystin and of Dihydrodemethylsterigmatocystin to Dihydrosterigmatocystin during Aflatoxin Biosynthesis.

Authors:  K Yabe; K Matsushima; T Koyama; T Hamasaki
Journal:  Appl Environ Microbiol       Date:  1998-01       Impact factor: 4.792

Review 4.  Oxidative Cyclization in Natural Product Biosynthesis.

Authors:  Man-Cheng Tang; Yi Zou; Kenji Watanabe; Christopher T Walsh; Yi Tang
Journal:  Chem Rev       Date:  2016-12-12       Impact factor: 60.622

5.  Enzymes in aflatoxin biosynthesis.

Authors:  J A Anderson
Journal:  World J Microbiol Biotechnol       Date:  1992-12       Impact factor: 3.312

Review 6.  Recent advancements in the biosynthetic mechanisms for polyketide-derived mycotoxins.

Authors:  Justin Huffman; Ryan Gerber; Liangcheng Du
Journal:  Biopolymers       Date:  2010-09       Impact factor: 2.505

7.  Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans.

Authors:  D W Brown; J H Yu; H S Kelkar; M Fernandes; T C Nesbitt; N P Keller; T H Adams; T J Leonard
Journal:  Proc Natl Acad Sci U S A       Date:  1996-02-20       Impact factor: 11.205

8.  Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin in aflatoxin biosynthesis.

Authors:  C D Skory; P K Chang; J Cary; J E Linz
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

9.  Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus.

Authors:  F Trail; P K Chang; J Cary; J E Linz
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792

10.  Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus.

Authors:  G A Payne; G J Nystrom; D Bhatnagar; T E Cleveland; C P Woloshuk
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792
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