| Literature DB >> 17314371 |
Isabelle Poilane1, Christel Humeniuk-Ainouz1, Isabelle Durand1, Claire Janoir2, Philippe Cruaud1, Michel Delmée3, Michel R Popoff4, Anne Collignon2,1.
Abstract
The discriminatory potential of a combination of various typing methods was evaluated on a set of 21 Clostridium difficile isolates obtained from symptomatic patients hospitalized in a geriatric unit and 7 non-toxigenic isolates from the same hospital. Isolates were firstly serotyped and toxinotyped. Of the 28 isolates, 19 belonged to serogroup A. PCR-ribotyping and PCR-RFLP on the fliC and slpA genes were then applied to these 19 isolates. The results suggest that the combination of PCR-ribotyping with PCR-RFLP analysis of slpA could be more discriminatory and suitable for studying C. difficile epidemiology.Entities:
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Year: 2007 PMID： 17314371 DOI： 10.1099/jmm.0.46608-0
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472