AIMS: To investigate the probiotic properties of Bacillus polyfermenticus SCD such as their adherence to Caco-2 cells and anticarcinogenic effects on human colon cancer cells and rat colon cancer carcinogenesis. METHODS AND RESULTS: The cell surface hydrophobicity and cell agglutination of B. polyfermenticus SCD was 64.04 +/- 1.25% and 297.3 +/- 8.7 mg ml(-1), respectively. This strain was strongly adherent to Caco-2 cells. Bacillus polyfermenticus SCD was also found to inhibit the growth of colon cancer cells in a dose-dependent manner as detected by the MTT assay. After 10 weeks of B. polyfermenticus SCD supplementation with 3 x 10(6) CFU day(-1) in F344 male rats, dimethylhydrazine-induced aberrant crypts and preneoplastic lesions decreased by 40% compared with the control rats. CONCLUSIONS: Bacillus polyfermenticus SCD has strong adherent properties in the colon, and anticarcinogenic effects in vitro and in vivo. SIGNIFICANCE AND IMPACT OF THE STUDY: These new findings on the characteristics of B. polyfermenticus SCD will be valuable in the evaluation of this commercial probiotic. Also, B. polyfermenticus SCD can be useful for the inhibition of colon cancer cells as an ingredient of medicinal foods or new drugs.
AIMS: To investigate the probiotic properties of Bacillus polyfermenticusSCD such as their adherence to Caco-2 cells and anticarcinogenic effects on humancolon cancer cells and ratcolon cancer carcinogenesis. METHODS AND RESULTS: The cell surface hydrophobicity and cell agglutination of B. polyfermenticusSCD was 64.04 +/- 1.25% and 297.3 +/- 8.7 mg ml(-1), respectively. This strain was strongly adherent to Caco-2 cells. Bacillus polyfermenticusSCD was also found to inhibit the growth of colon cancer cells in a dose-dependent manner as detected by the MTT assay. After 10 weeks of B. polyfermenticusSCD supplementation with 3 x 10(6) CFU day(-1) in F344 male rats, dimethylhydrazine-induced aberrant crypts and preneoplastic lesions decreased by 40% compared with the control rats. CONCLUSIONS:Bacillus polyfermenticusSCD has strong adherent properties in the colon, and anticarcinogenic effects in vitro and in vivo. SIGNIFICANCE AND IMPACT OF THE STUDY: These new findings on the characteristics of B. polyfermenticusSCD will be valuable in the evaluation of this commercial probiotic. Also, B. polyfermenticusSCD can be useful for the inhibition of colon cancer cells as an ingredient of medicinal foods or new drugs.
Authors: Elise L Ma; Yoon Jeong Choi; Jinyoung Choi; Charalabos Pothoulakis; Sang Hoon Rhee; Eunok Im Journal: Int J Cancer Date: 2010-08-15 Impact factor: 7.396