The combined effect of two aqueous extracts on the growth of Trichomonas vaginalis, in vitro.

In this study a trial was performed to determine the effect of a combination of two aqueous extracts of two medicinal plants namely the violet, Viola odorata, and the rue, Ruta graveolens, with concentrations of 0.15625, 0.3125, 10-20 mg/cm3 on the growth of Trichomonas vaginalis cultured in (CM161) medium during periods of 24, 48, 72, and 96 hrs. The results showed that there is a variation of inhibition. Complete inhibition was seen with a concentration 10 mg/cm3 for 48 hrs. During the 96 hr. Period, 81% inhibition was achieved at a concentration of 0.3125mg /cm3 , and 75% at a concentration of 0.15625mg/cm3. A variety of interaction was observed in combination of violet and rue. Significant synergism was achieved at a concentration of 20 mg/cm3, and a suggestive synergism was achieved with 10 mg /cm3 In addition, partial antagonism occurred at a concentration of 0.15625mg /cm3 and an antagonism at 0.3125mg /cm3 was achieved.