Literature DB >> 17307364

A novel cold-inducible expression system for Bacillus subtilis.

Ai Thi Thuy Le1, Wolfgang Schumann.   

Abstract

Production of recombinant proteins at low temperatures is one strategy to prevent formation of protein aggregates and the use of an expensive inducer such as IPTG. We report on the construction of two expression vectors both containing the cold-inducible des promoter of Bacillus subtilis, where one allows intra- and the other extracellular synthesis of recombinant proteins. Production of recombinant proteins started within the first 30min after temperature downshock to 25 degrees C and continued for about 5h.

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Year:  2007        PMID： 17307364     DOI： 10.1016/j.pep.2006.12.023

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  10 in total

1.  Stress-responsive systems set specific limits to the overproduction of membrane proteins in Bacillus subtilis.

Authors:  Jessica C Zweers; Thomas Wiegert; Jan Maarten van Dijl
Journal:  Appl Environ Microbiol       Date:  2009-10-09       Impact factor: 4.792

Review 2.  Enhanced Recombinant Protein Production Under Special Environmental Stress.

Authors:  Xinyi Chen; Chun Li; Hu Liu
Journal:  Front Microbiol       Date:  2021-04-15       Impact factor: 5.640

3.  Construction and development of an auto-regulatory gene expression system in Bacillus subtilis.

Authors:  Chengran Guan; Wenjing Cui; Jintao Cheng; Li Zhou; Junling Guo; Xu Hu; Guoping Xiao; Zhemin Zhou
Journal:  Microb Cell Fact       Date:  2015-09-21       Impact factor: 5.328

4.  Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli.

Authors:  Trang Thi Phuong Phan; Linh Thuoc Tran; Wolfgang Schumann; Hoang Duc Nguyen
Journal:  Microb Cell Fact       Date:  2015-05-21       Impact factor: 5.328

5.  Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes.

Authors:  Gro Elin Kjæreng Bjerga; Rahmi Lale; Adele Kim Williamson
Journal:  Bioengineered       Date:  2015-12-28       Impact factor: 3.269

6.  Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis.

Authors:  Chaoyang Zhou; Bin Ye; Shan Cheng; Leizhen Zhao; Yuanxin Liu; Jiandong Jiang; Xin Yan
Journal:  Microb Cell Fact       Date:  2019-06-14       Impact factor: 5.328

7.  An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation.

Authors:  Gang Fu; Jie Yue; Dandan Li; Yixin Li; Sang Yup Lee; Dawei Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2022-03-09       Impact factor: 12.779

8.  Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature.

Authors:  Teresa San-Miguel; Pedro Pérez-Bermúdez; Isabel Gavidia
Journal:  Springerplus       Date:  2013-03-08

9.  The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter.

Authors:  Anna A Toymentseva; Karen Schrecke; Margarita R Sharipova; Thorsten Mascher
Journal:  Microb Cell Fact       Date:  2012-10-30       Impact factor: 5.328

Review 10.  The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production.

Authors:  Albert Uhoraningoga; Gemma K Kinsella; Gary T Henehan; Barry J Ryan
Journal:  Bioengineering (Basel)       Date:  2018-10-19
  10 in total

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