| Literature DB >> 17306925 |
Jitka Poljaková1, Eva Frei, Jacobo E Gomez, Dagmar Aimová, Tomás Eckschlager, Jan Hrabeta, Marie Stiborová.
Abstract
Ellipticine induces formation of two DNA adducts in leukemia HL-60 and CCRF-CEM cells, identical with deoxyguanosine adducts generated by ellipticine metabolites 13-hydroxyellipticine and 12-hydroxyellipticine in vitro and in vivo. The ellipticine cytotoxicity to HL-60 (IC(50)=0.64microM) and CCRF-CEM cells (IC(50)=4.7microM) correlates with levels of DNA adducts. The different expressions of enzymes activating ellipticine in cells explain this finding. While cytochrome P450 1A1 and cyclooxygenase-1 are expressed in both cells, HL-60 cells express also high levels of another activator, myeloperoxidase. The results suggest the adduct formation as a new mode of antitumor action of ellipticine for leukemia.Entities:
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Year: 2007 PMID： 17306925 DOI： 10.1016/j.canlet.2006.12.037
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679