Synthesis of a fluorescence-labeled K30 antigen repeating unit using click chemistry.

An N-dansyl-labeled K30 antigen repeating unit, [4-[5-(N,N'-dimethylamino)naphthalene-1-sulfonamine]-1H-1,2,3-triazol-1-yl]hexyl beta-D-glucopyranosyluronate-(1-->3)-alpha-D-galactopyranosyl-alpha-D-mannopyranosyl-(1-->3)-beta-D-galactopyranoside, was synthesized using click chemistry, the copper(I)-catalyzed 1,3-dipolar cycloaddition reaction of an azide and an alkyne. The target compound could further facilitate the studies of interactions among K30 oligosaccharides and proteins.