PURPOSE: This study was designed to determine whether telomerase activity measured in samples of tumoral tissue, transitional mucosa, and normal mucosa from patients with sporadic colorectal cancer is a prognostic factor for recurrence and overall survival. METHODS: Telomerase activity was determined by fluorescence-based telomeric repeat amplification in tissue samples from 108 patients with sporadic colorectal cancer. A telomerase index was determined by using the formula log (telomerase activity of cancer tissue - telomerase activity of normal mucosa). RESULTS: Mean telomerase activity in tumoral tissue was 11.49 (total product generated), in transitional mucosa it was 1.51, and in normal mucosa it was 1.09 (P < 0.001). Telomerase activity and telomerase index were not correlated with clinicopathologic factors. Rectal cancer patients' recurrence-free survival was related to N classification (P = 0.004) and to tumor-node-metastases stage classification (P = 0.023) and telomerase index 0.85 (P = 0.023). Overall survival was associated with N classification (positive/negative) and telomerase index (</=0.85 or >0.85; P = 0.018 and P = 0.011, respectively). CONCLUSIONS: Measurement of telomerase activity has a diagnostic value in colorectal patients. In rectal cancer, telomerase index is an independent prognostic factor for disease progression. A telomerase index</=0.85 and negative nodes can be used to predict disease progression.
PURPOSE: This study was designed to determine whether telomerase activity measured in samples of tumoral tissue, transitional mucosa, and normal mucosa from patients with sporadic colorectal cancer is a prognostic factor for recurrence and overall survival. METHODS: Telomerase activity was determined by fluorescence-based telomeric repeat amplification in tissue samples from 108 patients with sporadic colorectal cancer. A telomerase index was determined by using the formula log (telomerase activity of cancer tissue - telomerase activity of normal mucosa). RESULTS: Mean telomerase activity in tumoral tissue was 11.49 (total product generated), in transitional mucosa it was 1.51, and in normal mucosa it was 1.09 (P < 0.001). Telomerase activity and telomerase index were not correlated with clinicopathologic factors. Rectal cancerpatients' recurrence-free survival was related to N classification (P = 0.004) and to tumor-node-metastases stage classification (P = 0.023) and telomerase index 0.85 (P = 0.023). Overall survival was associated with N classification (positive/negative) and telomerase index (</=0.85 or >0.85; P = 0.018 and P = 0.011, respectively). CONCLUSIONS: Measurement of telomerase activity has a diagnostic value in colorectalpatients. In rectal cancer, telomerase index is an independent prognostic factor for disease progression. A telomerase index</=0.85 and negative nodes can be used to predict disease progression.
Authors: Jason H Sakamoto; Anne L van de Ven; Biana Godin; Elvin Blanco; Rita E Serda; Alessandro Grattoni; Arturas Ziemys; Ali Bouamrani; Tony Hu; Shivakumar I Ranganathan; Enrica De Rosa; Jonathan O Martinez; Christine A Smid; Rachel M Buchanan; Sei-Young Lee; Srimeenakshi Srinivasan; Matthew Landry; Anne Meyn; Ennio Tasciotti; Xuewu Liu; Paolo Decuzzi; Mauro Ferrari Journal: Pharmacol Res Date: 2010-01-05 Impact factor: 7.658
Authors: Andrew J Pellatt; Roger K Wolff; Gabriela Torres-Mejia; Esther M John; Jennifer S Herrick; Abbie Lundgreen; Kathy B Baumgartner; Anna R Giuliano; Lisa M Hines; Laura Fejerman; Richard Cawthon; Martha L Slattery Journal: Genes Chromosomes Cancer Date: 2013-04-30 Impact factor: 5.006
Authors: Cristina Frías; Alberto Morán; Carmen de Juan; Paloma Ortega; Tamara Fernández-Marcelo; Andrés Sánchez-Pernaute; Antonio José Torres; Eduardo Díaz-Rubio; Manuel Benito; Pilar Iniesta Journal: World J Gastrointest Oncol Date: 2009-10-15
Authors: Andrew J Pellatt; Roger K Wolff; Jennifer Herrick; Abbie Lundgreen; Martha L Slattery Journal: Mol Carcinog Date: 2012-02-21 Impact factor: 4.784