OBJECTIVES: To investigate the association between tumor necrosis factor-alpha (TNF-alpha) G-308A, tumor necrosis factor-beta (TNF-beta) G+252A and interleukin-4 (IL-4) C-590T polymorphisms and susceptibility to multiple sclerosis (MS) development and clinical course of the disease. MATERIALS AND METHODS: Two hundred and seventy patients with MS and 542 sex and ethnic matched controls were enrolled in the present study. An allele-specific oligonucleotide polymerase chain reaction was used to detect the polymorphism at position -308 of the TNF-alpha gene. The genotypes of TNF-beta and IL-4 were determined by polymerase chain reaction-restriction fragment length polymorphism. RESULTS: Allelic and genotypic frequencies for these polymorphisms were similar in patients with MS and population controls or among different types of the disease. CONCLUSION: The results of the present study suggest that the three mentioned functional polymorphisms are not likely to cause susceptibility to MS in the Iranian population.
OBJECTIVES: To investigate the association between tumor necrosis factor-alpha (TNF-alpha) G-308A, tumor necrosis factor-beta (TNF-beta) G+252A and interleukin-4 (IL-4) C-590T polymorphisms and susceptibility to multiple sclerosis (MS) development and clinical course of the disease. MATERIALS AND METHODS: Two hundred and seventy patients with MS and 542 sex and ethnic matched controls were enrolled in the present study. An allele-specific oligonucleotide polymerase chain reaction was used to detect the polymorphism at position -308 of the TNF-alpha gene. The genotypes of TNF-beta and IL-4 were determined by polymerase chain reaction-restriction fragment length polymorphism. RESULTS: Allelic and genotypic frequencies for these polymorphisms were similar in patients with MS and population controls or among different types of the disease. CONCLUSION: The results of the present study suggest that the three mentioned functional polymorphisms are not likely to cause susceptibility to MS in the Iranian population.
Authors: A P Kallaur; S R Oliveira; A N C Simão; E R D de Almeida; H K Morimoto; J Lopes; L M Pelegrino; W L C J de Pereira; Daniele Frizon Alfieri; R M Andrade; S D Borelli; M A E Watanabe; D R Kaimen-Maciel; E M V Reiche Journal: J Mol Neurosci Date: 2014-04-04 Impact factor: 3.444
Authors: O Yu Makarycheva; E Yu Tsareva; M A Sudomoina; O G Kulakova; B V Titov; O V Bykova; N V Gol'tsova; L M Kuzenkova; A N Boiko; O O Favorova Journal: Acta Naturae Date: 2011-01 Impact factor: 1.845