Literature DB >> 1729400

Affinity purification of human tau proteins and the construction of a sensitive sandwich enzyme-linked immunosorbent assay for human tau detection.

M Mercken1, M Vandermeeren, U Lübke, J Six, J Boons, E Vanmechelen, A Van de Voorde, J Gheuens.   

Abstract

Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml.

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Year:  1992        PMID： 1729400     DOI： 10.1111/j.1471-4159.1992.tb09754.x

Source DB:  PubMed          Journal:  J Neurochem        ISSN： 0022-3042            Impact factor:   5.372


  12 in total

1.  Both total and phosphorylated tau are increased in Alzheimer's disease.

Authors:  M Sjögren; P Davidsson; M Tullberg; L Minthon; A Wallin; C Wikkelso; A K Granérus; H Vanderstichele; E Vanmechelen; K Blennow
Journal:  J Neurol Neurosurg Psychiatry       Date:  2001-05       Impact factor: 10.154

2.  Monoclonal antibodies with selective specificity for Alzheimer Tau are directed against phosphatase-sensitive epitopes.

Authors:  M Mercken; M Vandermeeren; U Lübke; J Six; J Boons; A Van de Voorde; J J Martin; J Gheuens
Journal:  Acta Neuropathol       Date:  1992       Impact factor: 17.088

3.  Tau protein concentrations in cerebrospinal fluid of patients with dementia of the Alzheimer type.

Authors:  R E Tato; A Frank; A Hernanz
Journal:  J Neurol Neurosurg Psychiatry       Date:  1995-09       Impact factor: 10.154

4.  Phosphorylation-dependent epitopes of neurofilament antibodies on tau protein and relationship with Alzheimer tau.

Authors:  B Lichtenberg-Kraag; E M Mandelkow; J Biernat; B Steiner; C Schröter; N Gustke; H E Meyer; E Mandelkow
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

5.  Specific monoclonal antibodies against normal microtubule-associated protein-2 (MAP2) epitopes present in Alzheimer pathological structures do not recognize paired helical filaments.

Authors:  J Six; U Lübke; M Mercken; M Vandermeeren; C Ceuterick; A Van de Voorde; J Boons; J Gheuens
Journal:  Acta Neuropathol       Date:  1992       Impact factor: 17.088

6.  Prophylactic treatment with paroxetine ameliorates behavioral deficits and retards the development of amyloid and tau pathologies in 3xTgAD mice.

Authors:  Rhonda L Nelson; Zhihong Guo; Veerendra Madala Halagappa; Michelle Pearson; Audrey J Gray; Yasuji Matsuoka; Martin Brown; Bronwen Martin; Titilola Iyun; Stuart Maudsley; Robert F Clark; Mark P Mattson
Journal:  Exp Neurol       Date:  2007-02-13       Impact factor: 5.330

7.  Microtubule-associated protein tau epitopes are present in fiber lesions in diverse muscle disorders.

Authors:  U Lübke; J Six; M Villanova; J Boons; M Vandermeeren; C Ceuterick; P Cras; J J Martin
Journal:  Am J Pathol       Date:  1994-07       Impact factor: 4.307

8.  Mitogen activated protein (MAP) kinase transforms tau protein into an Alzheimer-like state.

Authors:  G Drewes; B Lichtenberg-Kraag; F Döring; E M Mandelkow; J Biernat; J Goris; M Dorée; E Mandelkow
Journal:  EMBO J       Date:  1992-06       Impact factor: 11.598

9.  Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice.

Authors:  Cristian A Lasagna-Reeves; Diana L Castillo-Carranza; Urmi Sengupta; Audra L Clos; George R Jackson; Rakez Kayed
Journal:  Mol Neurodegener       Date:  2011-06-06       Impact factor: 14.195

10.  Transmission and spreading of tauopathy in transgenic mouse brain.

Authors:  Florence Clavaguera; Tristan Bolmont; R Anthony Crowther; Dorothee Abramowski; Stephan Frank; Alphonse Probst; Graham Fraser; Anna K Stalder; Martin Beibel; Matthias Staufenbiel; Mathias Jucker; Michel Goedert; Markus Tolnay
Journal:  Nat Cell Biol       Date:  2009-06-07       Impact factor: 28.824
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