Literature DB >> 17293533

A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.

Reindert Nijland1, Jan-Willem Veening, Oscar P Kuipers.   

Abstract

By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter.

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Year:  2007        PMID： 17293533      PMCID： PMC1855636          DOI： 10.1128/AEM.02266-06

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A.

Authors:  Masaya Fujita; Richard Losick
Journal:  Genes Dev       Date:  2005-09-15       Impact factor: 11.361

2.  Importance of spore mutants for fed-batch and continuous fermentation of Bacillus subtilis.

Authors:  M K Oh; B G Kim; S H Park
Journal:  Biotechnol Bioeng       Date:  1995-09-20       Impact factor: 4.530

3.  Site-directed mutagenesis of Clostridium perfringens beta-toxin: expression of wild-type and mutant toxins in Bacillus subtilis.

Authors:  V Steinthorsdottir; V Fridriksdottir; E Gunnarsson; O S Andrésson
Journal:  FEMS Microbiol Lett       Date:  1998-01-01       Impact factor: 2.742

4.  Identification, sequence, and expression of the gene encoding gamma-glutamyltranspeptidase in Bacillus subtilis.

Authors:  K Xu; M A Strauch
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

5.  Host/vector interactions which affect the viability of recombinant phage lambda clones.

Authors:  K F Wertman; A R Wyman; D Botstein
Journal:  Gene       Date:  1986       Impact factor: 3.688

6.  Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis.

Authors:  M Perego; G B Spiegelman; J A Hoch
Journal:  Mol Microbiol       Date:  1988-11       Impact factor: 3.501

7.  Heterologous production and secretion of Clostridium perfringens beta-toxoid in closely related Gram-positive hosts.

Authors:  Reindert Nijland; Cordula Lindner; Mariska van Hartskamp; Leendert W Hamoen; Oscar P Kuipers
Journal:  J Biotechnol       Date:  2006-07-27       Impact factor: 3.307

8.  Effects of phosphorelay perturbations on architecture, sporulation, and spore resistance in biofilms of Bacillus subtilis.

Authors:  Jan-Willem Veening; Oscar P Kuipers; Stanley Brul; Klaas J Hellingwerf; Remco Kort
Journal:  J Bacteriol       Date:  2006-04       Impact factor: 3.490

9.  Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay.

Authors:  D Burbulys; K A Trach; J A Hoch
Journal:  Cell       Date:  1991-02-08       Impact factor: 41.582

10.  Genome engineering reveals large dispensable regions in Bacillus subtilis.

Authors:  Helga Westers; Ronald Dorenbos; Jan Maarten van Dijl; Jorrit Kabel; Tony Flanagan; Kevin M Devine; Florence Jude; Simone J Seror; Aaron C Beekman; Elise Darmon; Caroline Eschevins; Anne de Jong; Sierd Bron; Oscar P Kuipers; Alessandra M Albertini; Haike Antelmann; Michael Hecker; Nicola Zamboni; Uwe Sauer; Claude Bruand; Dusko S Ehrlich; Juan C Alonso; Margarita Salas; Wim J Quax
Journal:  Mol Biol Evol       Date:  2003-08-29       Impact factor: 16.240
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