Literature DB >> 1729251

Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo.

I Iost1, J Guillerez, M Dreyfus.   

Abstract

We show that in Escherichia coli at 32 degrees C, the T7 RNA polymerase travels over the lacZ gene about eightfold faster than ribosomes travel over the corresponding mRNA. We discuss how the T7 phage might exploit this high rate in its growth optimization strategy and how it obviates the possible drawbacks of uncoupling transcription from translation.

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Year:  1992        PMID： 1729251      PMCID： PMC205757          DOI： 10.1128/jb.174.2.619-622.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  23 in total

1.  Transcription of single-copy hybrid lacZ genes by T7 RNA polymerase in Escherichia coli: mRNA synthesis and degradation can be uncoupled from translation.

Authors:  M Chevrier-Miller; N Jacques; O Raibaud; M Dreyfus
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

Review 2.  Preventing the synthesis of unused transcripts by Rho factor.

Authors:  J P Richardson
Journal:  Cell       Date:  1991-03-22       Impact factor: 41.582

3.  Transcription and decay of the lac messenger: role of an intergenic terminator.

Authors:  G J Murakawa; C Kwan; J Yamashita; D P Nierlich
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

Review 4.  Translation initiation in Escherichia coli: old and new questions.

Authors:  N Jacques; M Dreyfus
Journal:  Mol Microbiol       Date:  1990-07       Impact factor: 3.501

5.  Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli.

Authors:  J R McCormick; J M Zengel; L Lindahl
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

6.  Identification and characterization of transcription termination sites in the Escherichia coli lacZ gene.

Authors:  E C Ruteshouser; J P Richardson
Journal:  J Mol Biol       Date:  1989-07-05       Impact factor: 5.469

7.  Regulation of coliphage T3 and T7 RNA polymerases by the lac repressor-operator system.

Authors:  T J Giordano; U Deuschle; H Bujard; W T McAllister
Journal:  Gene       Date:  1989-12-14       Impact factor: 3.688

8.  T7 lysozyme inhibits transcription by T7 RNA polymerase.

Authors:  B A Moffatt; F W Studier
Journal:  Cell       Date:  1987-04-24       Impact factor: 41.582

9.  Vectors for selective expression of cloned DNAs by T7 RNA polymerase.

Authors:  A H Rosenberg; B N Lade; D S Chui; S W Lin; J J Dunn; F W Studier
Journal:  Gene       Date:  1987       Impact factor: 3.688

10.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469
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  47 in total

1.  Reversal of inhibition by the T7 concatemer junction sequence on expression from a downstream T7 promoter.

Authors:  L Cheng; E Goldman
Journal:  Gene Expr       Date:  2001

2.  RNA stem-loop enhanced expression of previously non-expressible genes.

Authors:  Michael Paulus; Martin Haslbeck; Manfred Watzele
Journal:  Nucleic Acids Res       Date:  2004-05-26       Impact factor: 16.971

3.  Inhibition of translation by consecutive rare leucine codons in E. coli: absence of effect of varying mRNA stability.

Authors:  Ping Shu; Huacheng Dai; Wenwu Gao; Emanuel Goldman
Journal:  Gene Expr       Date:  2006

4.  Question 6: early steps of evolution and some ideas about a simplified translational machinery.

Authors:  Knud H Nierhaus
Journal:  Orig Life Evol Biosph       Date:  2007-08-01       Impact factor: 1.950

5.  Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition.

Authors:  Paul G Blommel; Katie J Becker; Petar Duvnjak; Brian G Fox
Journal:  Biotechnol Prog       Date:  2007-05-17

6.  Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress.

Authors:  Thomas Baumgarten; A Jimmy Ytterberg; Roman A Zubarev; Jan-Willem de Gier
Journal:  Appl Environ Microbiol       Date:  2018-05-31       Impact factor: 4.792

7.  Tuning Escherichia coli for membrane protein overexpression.

Authors:  Samuel Wagner; Mirjam M Klepsch; Susan Schlegel; Ansgar Appel; Roger Draheim; Michael Tarry; Martin Högbom; Klaas J van Wijk; Dirk J Slotboom; Jan O Persson; Jan-Willem de Gier
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-16       Impact factor: 11.205

8.  An rne-1 pnp-7 double mutation suppresses the temperature-sensitive defect of lacZ gene expression in a divE mutant.

Authors:  T Aiso; R Ohki
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

9.  Translation inhibitors stabilize Escherichia coli mRNAs independently of ribosome protection.

Authors:  P J Lopez; I Marchand; O Yarchuk; M Dreyfus
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-26       Impact factor: 11.205

Review 10.  How bacterial cells keep ribonucleases under control.

Authors:  Murray P Deutscher
Journal:  FEMS Microbiol Rev       Date:  2015-04-14       Impact factor: 16.408
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