Literature DB >> 1729236

Purification of a nocardicin A-sensitive LD-carboxypeptidase from Escherichia coli by affinity chromatography.

A Ursinus1, H Steinhaus, J V Höltje.   

Abstract

An LD-carboxypeptidase releasing the terminal D-Ala from UDP-MurNAc-L-Ala-D-Glu-m-A2pm-D-Ala (UDP-MurNAc-tetrapeptide) was purified from Escherichia coli to biochemical homogeneity and characterized biochemically. Final purification was achieved by nocardicin A-Sepharose affinity chromatography. An apparent molecular weight of 32,000 was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the enzyme, which seems to be a monomeric protein as indicated by gel filtration. The optimum pH of the enzyme was 8.4, and the pI was 5.5. The Km for UDP-MurNAc-tetrapeptide was 1.5 x 10(-4) M, and the Vmax was 0.4 nmol/min. Nocardicin A inhibited the enzyme competitively, with a Ki of 5 x 10(-5) M. Benzylpenicillin, cephalosporin C, thienamycin, and D-alanyl-D-alanine did not affect the enzyme activity. Possible functions of the enzyme for growth and division of the murein sacculus are discussed.

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Year:  1992        PMID： 1729236      PMCID： PMC205735          DOI： 10.1128/jb.174.2.441-446.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

Review 1.  The murein hydrolases of Escherichia coli: properties, functions and impact on the course of infections in vivo.

Authors:  J V Höltje; E I Tuomanen
Journal:  J Gen Microbiol       Date:  1991-03

2.  Growth pattern of the murein sacculus of Escherichia coli.

Authors:  B Glauner; J V Höltje
Journal:  J Biol Chem       Date:  1990-11-05       Impact factor: 5.157

3.  Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.

Authors:  J H Morrissey
Journal:  Anal Biochem       Date:  1981-11-01       Impact factor: 3.365

4.  Spherical E. coli due to elevated levels of D-alanine carboxypeptidase.

Authors:  Z Markiewicz; J K Broome-Smith; U Schwarz; B G Spratt
Journal:  Nature       Date:  1982-06-24       Impact factor: 49.962

5.  The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide.

Authors:  K J Begg; A Takasuga; D H Edwards; S J Dewar; B G Spratt; H Adachi; T Ohta; H Matsuzawa; W D Donachie
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

6.  Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition.

Authors:  B Korat; H Mottl; W Keck
Journal:  Mol Microbiol       Date:  1991-03       Impact factor: 3.501

7.  LD-carboxypeptidase activity in Escherichia coli. I. The LD-carboxypeptidase activity in ether treated cells.

Authors:  R Metz; S Henning; W P Hammes
Journal:  Arch Microbiol       Date:  1986-03       Impact factor: 2.552

8.  LD-carboxypeptidase activity in Escherichia coli. II. Isolation, purification and characterization of the enzyme from E. coli K 12.

Authors:  R Metz; S Henning; W P Hammes
Journal:  Arch Microbiol       Date:  1986-03       Impact factor: 2.552

9.  One-step purification procedure for UDP-N-acetylmuramyl-peptide murein precursors from Bacillus cereus.

Authors:  U Kohlrausch; J V Höltje
Journal:  FEMS Microbiol Lett       Date:  1991-03-01       Impact factor: 2.742

10.  Penicillin target enzyme and the antibiotic binding site.

Authors:  J A Kelly; P C Moews; J R Knox; J M Frère; J M Ghuysen
Journal:  Science       Date:  1982-10-29       Impact factor: 47.728
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  11 in total

Review 1.  Biochemistry and comparative genomics of SxxK superfamily acyltransferases offer a clue to the mycobacterial paradox: presence of penicillin-susceptible target proteins versus lack of efficiency of penicillin as therapeutic agent.

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Journal:  Microbiol Mol Biol Rev       Date:  2002-12       Impact factor: 11.056

2.  Probing the catalytic activity of a cell division-specific transpeptidase in vivo with beta-lactams.

Authors:  Christian Eberhardt; Lars Kuerschner; David S Weiss
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

3.  Structure and function of a novel LD-carboxypeptidase a involved in peptidoglycan recycling.

Authors:  Debanu Das; Mireille Hervé; Marc-André Elsliger; Rameshwar U Kadam; Joanna C Grant; Hsiu-Ju Chiu; Mark W Knuth; Heath E Klock; Mitchell D Miller; Adam Godzik; Scott A Lesley; Ashley M Deacon; Dominique Mengin-Lecreulx; Ian A Wilson
Journal:  J Bacteriol       Date:  2013-10-11       Impact factor: 3.490

Review 4.  Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli.

Authors:  J V Höltje
Journal:  Microbiol Mol Biol Rev       Date:  1998-03       Impact factor: 11.056

5.  Identification of a dithiazoline inhibitor of Escherichia coli L,D-carboxypeptidase A.

Authors:  Ellen Z Baum; Steven M Crespo-Carbone; Barbara Foleno; Sean Peng; Jamese J Hilliard; Darren Abbanat; Raul Goldschmidt; Karen Bush
Journal:  Antimicrob Agents Chemother       Date:  2005-11       Impact factor: 5.191

Review 6.  From growth to autolysis: the murein hydrolases in Escherichia coli.

Authors:  J V Höltje
Journal:  Arch Microbiol       Date:  1995-10       Impact factor: 2.552

7.  Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis.

Authors:  S A Denome; P K Elf; T A Henderson; D E Nelson; K D Young
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

8.  Selective Inhibition of Neisseria gonorrhoeae by a Dithiazoline in Mixed Infections with Lactobacillus gasseri.

Authors:  Jonathan D Lenz; Kristina A Shirk; Adrienne Jolicoeur; Joseph P Dillard
Journal:  Antimicrob Agents Chemother       Date:  2018-11-26       Impact factor: 5.191

9.  Characterization of structural variations in the peptidoglycan of vancomycin-susceptible Enterococcus faecium: understanding glycopeptide-antibiotic binding sites using mass spectrometry.

Authors:  Gary J Patti; Jiawei Chen; Jacob Schaefer; Michael L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2008-07-01       Impact factor: 3.109

Review 10.  How bacteria consume their own exoskeletons (turnover and recycling of cell wall peptidoglycan).

Authors:  James T Park; Tsuyoshi Uehara
Journal:  Microbiol Mol Biol Rev       Date:  2008-06       Impact factor: 11.056
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